Figure 5

Thioredoxin oxidation reactions can affect each other. In (A), the flux through the thioredoxin-dependent reduction reaction of a substrate (PSS) at varying concentrations of a second thioredoxin substrate (RSS, Scheme II) was monitored in a kinetic model of the thioredoxin system (Scheme II). The concentrations of RSS were 0.1 (black, •••), 1 (red, --), 2 (blue, -) and 10 (green, -•-). In (B), the flux through the thioredoxin-dependent reduction of PSS with increasing concentrations of RSS was monitored over a range of thioredoxin reductase concentrations: 0.1 (black, •••), 1 (red, --), 2 (blue, -) and 10 (green, -•-). Parameters were as in Table 2, except where indicated otherwise. This figure was generated using 'Logic_Figure 5.psc' (Additional file 4). A SBML version of this model ('Logic_Figure 5.xml') is available as Additional file 8.