A summary of conditions that favor off-target effects in the n = 3 and the extended n = 3 networks. The conditions that promoted off-target effects in our model are summarized for two network types. Off-target effects in (A) the n = 3 network and (B) the extended n = 3 network were favored when cycle 3 tended toward deactivation and cycle 1 tended toward activation. Off-target effects were favored in cycle 4 of (B) the extended n = 3 network when cycle 2 tended toward deactivation and cycle 4 tended toward activation. Blue arrows in a cycle indicate which Vmax is larger when off-target effects are favored. (C) A summary of the specific conditions in each cycle found to favor off-target effects in the n = 3 network, in the extended n = 3 network, or in both networks is also provided.