A. Breakdown of the genes in iPC815 by COG classification. The COG classifications that make up the Miscellaneous category are: K, L, Q, S, T, U, and V. B. Venn diagrams showing the overlap between iPC815 and published E. coli, S. Typhimurium, and Y. pestis 91001 reconstructions based on shared versus organism-specific reactions (E. coli and S. Typhimurium) and E.C. numbers (Y. pestis 91001). In the latter case, an E.C. number associated with more than one reaction was only counted once. The low overlap between iPC815 and the Y. pestis 91001 reconstruction arises from the use of E.C. numbers to compare the two models rather than true biological differences between the two strains.