Systemic remodeling of the redox regulatory network due to RNAi perturbations of glutaredoxin 1, thioredoxin 1, and glucose-6-phosphate dehydrogenase

Table 2 Cell-line specific parameter sets for computational modeling

Initial condition/rate constant	Jurkat	pLKO	Grx1 shRNA	Trx1 shRNA	G6PD shRNA
Grx1-SH (μM)	1.2	1.2	0.62	1.2	1.2
Trx1_ox + Trx1_red (μM)	0.505	0.505	0.505	0.144	0.505
G6PD (M⋅s^-1)	3.75e-4	3.75e-4	2.55e-4	3.75e-4	1.24e-4
Prx2-SH₂ (μM)	19.2	19.2	12.5	17.1	16.7
Srx1 (s^-1)	3e-3	3e-3	1.74e-3	1.95e-3	3e-3
CAT (μM)	0.9	1.17	0.8	0.9	0.9

Numbers in bold represent altered parameters from the wild-type Jurkat cell line.

ISSN: 1752-0509