Formation of VEGF isoform-specific spatial distributions governing angiogenesis: computational analysis

BMC Systems Biology

Table 2 Kinetic Parameters

Isoforms Involved	Reaction	Forward k_on, k_c, k_int	Reverse k_off, k_uc	K_eq	Ref.
	*Cell-surface Reactions*
121, 165	Binding VEGF to VEGFR2	1·10⁷ M^-1s^-1	0.001 s^-1	100 pM	[42]
All	Binding VEGF to VEGFR1	3·10⁷ M^-1s^-1	0.001 s^-1	33.3 pM	[42]
165, 189	Binding VEGF to NRP1	3.2·10⁶ M^-1s^-1	0.001 s^-1	312 pM	[42]
165	Binding VEGF₁₆₅-VEGFR2 to NRP1	3.1·10⁶ [10¹⁵ μm²/(mol·s)]	0.001 s^-1	323 mol/10¹⁵ μm²	[42]
165	Coupling VEGF₁₆₅-NRP1 to VEGFR2	1·10⁷ [10¹⁵ μm²/(mol·s)]	0.001 s^-1	100 mol/10¹⁵ μm²	[42]
121, Cleaved	Coupling VEGFR1 to NRP1 or Coupling VEGF₁₁₄-VEGFR1 to NRP1	1·10⁷ [10¹⁵ μm²/(mol·s)]	0.01 s^-1	1000 mol/10¹⁵ μm²	[42]
-	Internalization, k_int	2.8·10^-4 s^-1	-	-	[42]
-	Insertion, s_R2, s_R1, or s_N1	e.g., k_int.·[R2]^total	-	-	calculated
	*ECM Reactions*
165	VEGF₁₆₅ to HSPG	6.06·10⁴ M^-1s^-1	0.01 s^-1	165 nM	[63]
189	VEGF₁₈₉ to HSPG	1.18·10⁶ M^-1s^-1	0.01 s^-1	8.5 nM	Figure S1.1
All	Degradation rate, k_deg (only Figs. 4C,D; 8A)	0.001 s^-1	-	-	assumed
All	Protease activity, k_P (only Figs. 6; 7A; 8A)	2.8·10^-4 s^-1	-	-	assumed

ISSN: 1752-0509