Figure 5

Simulations and lab results experiment #7. A: Reprint of Figure 2B in [20] (License agreement number 2773700432120): Kinetics of MITF and PIAS3 association in TPA-activated BL6-B16 cells. Coimmunoprecipitation (IP) of PIAS3 with MITF at defined time points in TPA-activated BL6-B16 cells. The cells were transfected with pcDNA-MITF and pcDNAPIAS3. The blots were probed with anti-MITF antibodies and then reprobed with anti-PIAS3 antibody. Phosphorylation states represented by the upper band are unknown, the lower band is anticipated to represent un-phosphorylated MITF. B: Simulation of the same experiment (experiment #7 in Table 2). The temporal development of the amount of MITF-PIAS3 complex is plotted for all phosphorylation states of MITF, as well as for the sum of the phosphorylated states (PIAS3-MITFpAll).