Identification of a 5-gene network without noise. (a) Convergence study of the genetic algorithm. The number of connections identified in each of the solutions generated by GA is plotted. No feasible solution was found with less than 65 generations. (b) Identified network. Arrows represent positive regulation and the filled circles represent negative regulation of the genes. Kinetic orders of each connection are represented above the corresponding connecting lines and the rate constants for each gene are shown above the genes. All connections and parameters are consistent with the original differential equations used to generate the in silico data.