Figure 9

Transient phosphorylation of phosphoinositides due to enzymatic complex formation. Comparison of the two scenarios; solid line represent the case when a signalling trigger is simulated. Parameters used are the following: λ₋₁ = 10 s⁻¹,λ₁ = 1μ M⁻¹s⁻¹,k₁ = 1 s⁻¹,λ₋₂ = 10 s⁻¹,λ₂ = 1μ M⁻¹s⁻¹,k₂ = 1 s⁻¹,κ₋₁=50s⁻¹,κ₁ = 1μ M⁻¹s⁻¹,K _t = 0. 1μ M,P _t = 0. 2μ M,S _t = 300μ M. The other parameters are λ₋₄ = 1 s⁻¹,λ₄ = 75μ M⁻¹s⁻¹,k₄ = 100 s⁻¹for t≤300 s and λ₋₃ = 10 s⁻¹,λ₃ = 10μ M⁻¹s⁻¹,k₃ = 500 s⁻¹, for t>300s. Dashed line represent the case when kinases are recruited linearly in time as K _t = 0. 0003t. Other parameters used are the following: λ₋₁ = 10 s⁻¹,λ₁ = 1μ M⁻¹s⁻¹,k₁ = 1 s⁻¹,λ₋₂ = 10 s⁻¹,λ₂ = 1μ M⁻¹s⁻¹,k₂ = 1 s⁻¹,κ₋₁ = 50 s⁻¹,κ₁ = 1μ M⁻¹s⁻¹,λ₋₃ = 10 s⁻¹,λ₃ = 10μ M⁻¹s⁻¹,k₃ = 500 s⁻¹,P _t = 0. 2μ M,S _t = 300μ M.