Figure 8
From: Modeling of human factor Va inactivation by activated protein C
Effect of Prothrombinase Components on APC Proteolysis of FVa. Normalized densitometry data measuring the disappearance of the FVaHC are from reactions containing 20 μM PC:PS, 2.0 nM APC and 20 nM FVa (filled circles, N = 6), with either 30 nM active site blocked FXa (open circles, N = 5), 1.4 μM prothrombin (filled triangles, N = 4), or 30 nM active site blocked FXa + 1.4 μM prothrombin (open triangles, N = 4). Solid lines are fits of the empirical data to monophasic exponential decay curves (FVa, FVa + FXa*, and FVa + PT) or a linear decay (FVa + FXa* + PT).