The split carbon metabolism of M145 grown on D-glucose and/or L-glutamate. (A) Summary of on-line and off-line analyses of fermentations performed in the 200 mL scale on 13 C6 D-glucose and non-labelled L-glutamate (ferm. #25, red), and on non-labelled substrates only (ferm. #24, blue), reference fermentation 1.8 L standard volume (ferm. #23, grey). (B) Schematic representation of parts of the central carbon metabolism including the two carbon sources used, and results from metabolite profiling and summed fractional labelling (SFL) determination (grey boxes) of selected metabolites (green/blue); Only the most significant SFLs are displayed, obtained by analysis of GC-MS data based on samples from ferm. #24 and #25 28 h (growth phase, left) and 44 h (transition phase, right) after inoculation. Relative % standard deviations are given in brackets below the SFLs. (C) M145 culture growth and antibiotic production in the absence of D-glucose (ferm. #22, red), or with L-glutamate (ferm. #20, grey) or ammonium (ferm. #21, blue) as the sources of nitrogen. For the latter, no significant growth was detected, not giving rise to any significant production phenotype.