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Table 2 Carbon consumption and derived secondary metabolite production yields in the time period 45–50 hrs

From: Optimized submerged batch fermentation strategy for systems scale studies of metabolic switching in Streptomyces coelicolor A3(2)

Ferm. #

Carbon source consumption

Yields [%Cmol]

D-glucose

L-glutamate

RED

γ-Act

TBP

Cmol/L-h

Cmol/L-h

Cmol RED/Cmol C source

Cmol ACT/Cmol C source

Cmol TBP/Cmol C source

18a

0.0059

0.0238

1.0

0.4

1.0

19a

0.0083

0

0.1

2.2

4.9

  1. Carbon consumption and derived secondary metabolite production yields determined for one representative cultivation each of experiments with high resolution time-series sampling for full-scale ‘omics analysis using the fully developed media and conditions for studying the effect of phosphate (ferm. #18a) and L-glutamate depletion (ferm. #19a), respectively, on metabolic switching by strain M145. Results from ‘omics analyses partly presented earlier [1922] or to be published elsewhere.