|
Gene
|
Position
|
Strand
|
Sequence
|
k
a
(M-1s-1)
|
k
d
(s-1)
|
K
D
(nM)
|
|---|
|
srbA
|
-735 to -726
|
Sense
|
ATCATACGAT
|
1.48 ± 0.05 * 107
|
9.27 ± 0.29 * 10-3
|
0.63 ± 0.04
|
|
srbA*
|
-735 to -726
|
Sense
|
ATATAACATA
|
5.92 ± 0.08 * 105
|
2.29 ± 0.01* 10-1
|
386 ± 7.3
|
|
hapX
|
-774 to -783
|
Antisense
|
ATCCTCCCAT
|
5.68 ± 0.07 * 105
|
1.61 ± 0.01* 10-1
|
282 ± 5.1
|
|
hapX
|
-1340 to -1331
|
Sense
|
ATCAGATGAT
|
3.10 ± 0.01 * 107
|
1.44 ± 0.01 * 10-1
|
4.64 ± 0.03
|
|
hemA
|
-527 to -518
|
Sense
|
ATCGGATCAT
|
1.41 ± 0.07 * 107
|
5.88 ± 0.28 * 10-2
|
4.18 ± 0.41
|
|
hemA
|
-338 to -347
|
Antisense
|
ATCGCCTCAT
|
1.03 ± 0.01 * 106
|
3.66 ± 0.02 * 10-2
|
35.7 ± 0.52
|
- The binding of SrbA to five predicted binding sites was analysed. Results indicate three high-affinity binding sites in promoter regions of hapX, hemA and srbA. The asterix denotes a mutated binding site. Mutated nucleotides are underlined. k
a
= association rate constant, k
d
= dissociation rate constant, K
D
= k
d
/k
a
equilibrium dissociation constant.
