Skip to main content

Table 4 SrbA binding sites

From: Regulatory interactions for iron homeostasis in Aspergillus fumigatus inferred by a Systems Biology approach

Gene Position Strand Sequence k a (M-1s-1) k d (s-1) K D (nM)
srbA -735 to -726 Sense ATCATACGAT 1.48 ± 0.05 * 107 9.27 ± 0.29 * 10-3 0.63 ± 0.04
srbA* -735 to -726 Sense ATATAACATA 5.92 ± 0.08 * 105 2.29 ± 0.01* 10-1 386 ± 7.3
hapX -774 to -783 Antisense ATCCTCCCAT 5.68 ± 0.07 * 105 1.61 ± 0.01* 10-1 282 ± 5.1
hapX -1340 to -1331 Sense ATCAGATGAT 3.10 ± 0.01 * 107 1.44 ± 0.01 * 10-1 4.64 ± 0.03
hemA -527 to -518 Sense ATCGGATCAT 1.41 ± 0.07 * 107 5.88 ± 0.28 * 10-2 4.18 ± 0.41
hemA -338 to -347 Antisense ATCGCCTCAT 1.03 ± 0.01 * 106 3.66 ± 0.02 * 10-2 35.7 ± 0.52
  1. The binding of SrbA to five predicted binding sites was analysed. Results indicate three high-affinity binding sites in promoter regions of hapX, hemA and srbA. The asterix denotes a mutated binding site. Mutated nucleotides are underlined. k a = association rate constant, k d = dissociation rate constant, K D = k d /k a equilibrium dissociation constant.