Figure 2

Activation of type I IFN pathway by IFN-beta in Raw cell line and the corresponding simulations from the ODE model. A-C) pSTAT1, STAT1 and SOCS1 protein concentration in cell lysates were measured by Luminex, Flow cytometry or ELISA after stimulation with IFN-beta (1000 units/ml). The data was normalized to the maximum level. A) pSTAT1 protein concentration in the Raw cells stimulated with IFN-beta; (B) Total STAT1 protein concentration in the Raw cells stimulated with IFN-beta; C) SOCS1 protein concentration in the Raw cells stimulated with IFN-beta. We plot here one out of three independent experiments, which were performed in duplicates. D-F) Model simulation and sensitivity analysis with ± 20% change for all parameters (shaded areas) (D: pSTAT1; E: STAT1; F: SOCS1).