From: Dynamics of the quorum sensing switch: stochastic and non-stationary effects
Parameter | Description | Value | Reference |
---|---|---|---|
K _{d 1} | dissociation constant of LuxR to A | 100 nM | [41] |
{k}_{1}^{-} | unbinding rate of LuxR to A | 10 mi n^{−1} | estimated |
K _{d 2} | dissociation constant of LuxR·AI dimerization | 20 nM | fitted |
{K}_{2}^{-} | dissociation rate of dimer (LuxR·AI)_{2} | 1 mi n^{−1} | estimated |
k _{ A } | synthesis rate of A by LuxI | 0.04 mi n^{−1} | fitted |
K _{ dlux } | dissociation constant of (LuxR·AI)_{2} to the lux promoter | 200 nM | fitted |
{k}_{\mathit{\text{lux}}}^{-} | dissociation rate of (LuxR·AI)_{2} to the lux promoter | 10 mi n^{−1} | estimated |
b | burst size | 20 | [38] |
k _{ R } | transcription rate of luxR | 200/b mi n^{−1} | fitted |
k _{ I } | transcription rate of luxI | 50/b mi n^{−1} | fitted |
p _{ R } | translation rate of luxR mRNA | b d _{ mR } mi n ^{−1} | |
p _{ I } | translation rate of luxI mRNA | b d _{ mI } mi n ^{−1} | |
α _{ R } | ratio between unactivated and activated rate of expression of luxR | 0.001 | fitted |
α _{ I } | ratio between unactivated and activated rate of expression of luxI | 0.01 | fitted |
d _{ A } | degradation rate of A (same inside and outside the cell) | 0.001 mi n^{−1} | [42] |
{d}_{{C}_{2}} | degradation rate of (LuxR·AI)_{2} | 0.002 mi n^{−1} | estimated |
d _{ C } | degradation rate of LuxR·AI | 0.002 mi n^{−1} | estimated |
d _{ R } | degradation rate of LuxR | 0.002 mi n^{−1} | estimated |
d _{ I } | degradation rate of LuxI | 0.01 mi n^{−1} | estimated |
d _{ mR } | degradation rate of luxR mRNA | 0.347 mi n^{−1} | [43] |
d _{ mI } | degradation rate of luxI mRNA | 0.347 mi n^{−1} | [43] |
D | effective diffusion rate of A through the cell membrane | 10 mi n^{−1} | [44] |
τ | cell cycle duration (doubling time) in RM/succinate at 30 C | 45 min | [10] |
λ | relative weight between the det./sto. components of the cell cycle | 0.8 | [33, 45] |
V _{0} | cell volume at the beginning of cell cycle | 1.5 μ m^{3} | [46] |
V _{ tot } | total cell culture volume | 2·10^{−4}μl |