Figure 3

Transcript levels of genes encoding putative NADPH-generating enzymes in R. sphaeroides under aerobic respiratory conditions. Transcript levels for genes encoding enzymes predicted by iRsp1095 to be involved in NADPH generation (Table 3), were assayed via qRT-PCR in the presence or absence of PntAB. (A) RSP_0240 (pntA) (B) RSP_1939 (NADPH-ferredoxin reductase) (C) RSP_2734 (zwf) (D) RSP_0446 (icd) (E) RSP_1217 (Malic enzyme) (F) RSP_0661 (5,10-methylene-tetrahydrofolate dehydrogenase) (G) RSP_1146 (Glutamate synthase) (H) RSP_1593 (Malic enzyme). Transcript levels in WT cells is shown in blue bars, while those for PntA1 is in red bars. All fold change values represent expression relative to WT succinate-grown cells, whose relative expression was set to 1. ** Significantly increased expression in PntA1 relative to WT.