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Figure 8 | BMC Systems Biology

Figure 8

From: Identifying reaction modules in metabolic pathways: bioinformatic deduction and experimental validation of a new putative route in purine catabolism

Figure 8

Analysis of the purified Rxyl_2847 enzyme. (A) SDS-PAGE of purified Rxyl_2847 enzyme on a NuPage Bis-Tris 4-12% gel (Life Technologies) in MOPS running buffer. Proteins were stained with PageRuler protein staining solution (ThermoScientific). Lane 1, PageRuler Unstained Broad Range Protein Ladder (ThermoScientific); Lane 2, 10Ā Ī¼g of purified Rxyl_2847 enzyme. (B) Western blot analysis of purified Rxyl_2847 enzyme (same condition of electrophoresis as in (A), electroblot onto Hybond (GE Healthcare) nitrocellulose membrane). Lane 1, PageRuler Unstained Broad Range Protein Ladder (ThermoScientific); Lane 2, 100Ā ng of purified Rxyl_2847 enzyme; Lane 3, 100Ā ng of a purified His-tagged protein control; Lane 4, 100Ā ng of a purified untagged protein control.

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BMC Systems Biology

ISSN: 1752-0509

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