Analysis of the purified Rxyl_2847 enzyme. (A) SDS-PAGE of purified Rxyl_2847 enzyme on a NuPage Bis-Tris 4-12% gel (Life Technologies) in MOPS running buffer. Proteins were stained with PageRuler protein staining solution (ThermoScientific). Lane 1, PageRuler Unstained Broad Range Protein Ladder (ThermoScientific); Lane 2, 10 μg of purified Rxyl_2847 enzyme. (B) Western blot analysis of purified Rxyl_2847 enzyme (same condition of electrophoresis as in (A), electroblot onto Hybond (GE Healthcare) nitrocellulose membrane). Lane 1, PageRuler Unstained Broad Range Protein Ladder (ThermoScientific); Lane 2, 100 ng of purified Rxyl_2847 enzyme; Lane 3, 100 ng of a purified His-tagged protein control; Lane 4, 100 ng of a purified untagged protein control.