Figure 1

The structures of the three Mus musculus Rnf14 transcript variants and their encoded proteins. Numbers denote exons. The binding sites for the three Agilent probes, ILMN_2675078, ILMN_2682811 and ILMN_2868579 are indicated. Also highlighted are the qRT-PCR primers designed to amplify all Rnf14 species including the transfection construct derived transcripts, and the primer set for amplifying endogenous Rnf14 only. The forward primers for amplifying the transfection construct derived transcripts are indicated; the location of reverse primer is not shown as it was designed in vector sequence downstream of the stop codon in exon 11. The dotted red lines denote beginning and the end of the Open Reading Frames. The * denotes the position of the first base of the amplified sequence inserted into the two transfection expression constructs. Solid blue lines indicate location and size of sequence inserted into the expression constructs. The RWD domain present on isoform A (translated from the variant 1 transcript) but not isoform B (translated from the variant 3 transcript) is also highlighted. The Rnf14 variant 2 transcript does not give rise to a protein.