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Table 1 Copy numbers of the vector and the TEV plasmid in CDC20 TIPI-gTOW in the gene-deletion strains

From: Evaluation of the lower protein limit in the budding yeast Saccharomyces cerevisiae using TIPI-gTOW

 

Vector copy number*

TEV plasmid copy number*

TEV/vector ratio

Relative change in copy number

Average

SD

Average

SD

Wild type

149.7

43.4

59.8

19.9

0.40

–

bck2Δ

44.3

3.4

25.3

0.8

0.57

0.43

bub2Δ

71.4

8.4

48.6

3.5

0.68

0.70

cdh1Δ

66.7

5.7

19.6

2.2

0.29

-0.27

cin8Δ

53.7

20.0

37.1

5.0

0.69

0.73

clb1Δ

57.6

5.2

19.2

2.2

0.33

-0.16

clb2Δ

56.3

4.3

20.2

7.8

0.36

-0.10

clb3Δ

77.5

15.1

27.7

3.6

0.36

-0.11

clb4Δ

59.4

4.5

19.3

2.1

0.32

-0.19

clb5Δ

98.0

21.4

33.6

6.5

0.34

-0.14

clb6Δ

76.1

8.6

31.6

2.7

0.42

0.04

cln1Δ

80.1

24.3

20.7

0.6

0.26

-0.35

cln2Δ

82.7

15.8

42.9

3.8

0.52

0.30

cln3Δ

47.0

10.9

22.0

10.8

0.47

0.17

lte1Δ

84.8

20.9

32.0

16.0

0.38

-0.06

mad2Δ

47.4

6.0

35.2

2.9

0.74

0.86

mbp1Δ

21.6

11.4

15.0

2.1

0.69

0.73

mih1Δ

64.4

12.1

29.3

3.7

0.46

0.14

sic1Δ

71.8

20.1

20.2

11.8

0.28

-0.29

swe1Δ

124.4

13.0

42.2

5.3

0.34

-0.15

swi4Δ

76.1

15.1

28.4

6.6

0.37

-0.07

swi5Δ

94.0

23.8

37.3

12.6

0.40

-0.01

swi6Δ

64.0

8.7

35.2

7.2

0.55

0.38

whi5Δ

69.6

15.8

33.8

10.9

0.49

0.22

  1. *Average and SD of four independent experimental trials are shown.