Figure 1

Overview of the experimental and computational procedures. Expression profiles of endothelial cells were taken after treatment in four environments and their expression was normalized by using three probabilistic methods that exploit the difference in the distributions of the perfectly matched and mismatched probes. Then we built the gene ontology and functional networks that we overlapped with the known PPI network, which is available in literature, to identify implicated processes and genes. To uncover putative TF-gene interactions that take place under these conditions, we used a supervised network inference approach that utilizes known information about TF genes and their targets to find similar expression patterns in our data and thus uncover novel interactions. Finally, top ranked over/under expressions are validated by qRT-PCR and their relative levels are quantified.