Figure 1

Cell morphology (top) and formation of actin filaments (bottom) of MG-63 osteoblasts on planar (left) and geometrically micro-pillared (right) titanium surfaces after 24 h. On the planar surface cells are closely attached to the surface with their entire cell body and exhibit a flattened phenotype. On the pillared surface P-5 × 5 × 5, cells are elongated and their adhesion is mainly restricted to the surface plateaus (FE-SEM Supra 25, Carl Zeiss; bar = 10 μm), image in cooperation with Regina Lange, Institute of Electronic Appliances and Circuits, University of Rostock). Long and well defined filaments actin filaments form in cells on planar surfaces, whereas on the pillared structure P-5 × 5 × 5 the actin filaments are accumulated on top on the pillars in short fibers (LSM 410, Carl Zeiss, green: reflexion mode from the surface, red: phalloidin-TRITC for actin) [9].