| Â |
in vitro[9] |
in silico (cf. Figure 9) |
|---|
| Â | Â | low angle deviation | high angle deviation |
|---|
| Â | Â | low severing | high severing |
|---|
|  | Ref | P-5 × 5 × 5 | planar | pillar | planar | pillar |
|---|
Average filament | Â |
length μm) | 9.7±1.5 | 3.1±1.5 |
1.09±.03
| 0.90±.03 | 0.64±.03 |
0.55±.03
|
Maximum filament | Â |
length (μm) | 51.5±11.9 | 6.7±2.0 |
3.03±.33
| 2.86±.17 | 1.82±.35 |
1.77±.18
|
Orientation | Â |
dispersion (%) | 66±14 | 84±10 |
36±1
| 36±1 | 65±1 |
65±1
|
- Ref and P-5 × 5 × 5 refer to wet-lab experiments for planar and pillared surfaces (taken from ([9], Table one); p <0.001 for the changes in all three quantified properties; averages and standard deviation of 30 cells per specimen). The final four columns refer to the simulation experiments (averages and standard deviation of seven simulation runs for each parameter combination; filament length calculated based on an actin diameter of 0.05 μm). The two columns in bold there best reflect the changes of conditions for the actual cell. Changes in average filament length significant at p <0.001 planar vs. pillar with same severing agent amount and angle deviation, all changes significant when comparing the first and last experiment column. Note that length values in vitro and in silico are not comparable - the FilaQuant software processing florescence microscopy images has cutoff parameters (regarding length and thickness of lines to consider a filament) while for the simulation every integrin/focal adhesion with at least one bound actin is considered a filament.
