Comparison of in silico predicted protein masses verses in vivo measurements for reconstructed content specific to i JL1678-ME. Bar graphs showing simulation results (pink) of protein levels from the reconstructed i JL1678-ME verses measured in vivo expression levels (blue) using averaged RNA-seq as a proxy for protein production. Results were taken from glucose M9 minimal media conditions. (A) Translocase protein levels. (B) Percentage of protein mass in each of the four compartments.