The effects of inhibiting SecA on growth rate. (A) A scatterplot showing the effects of decreasing enzyme efficiency of several key enzymes involved in Sec translocation (ATPase SecA, the channel SecYEGDF, and ribosome) have on growth rate. The growth rate was predicted by decreasing turnover rate (i.e., kcat) of SecA, SecYEGDF, and ribosome and optimizing for growth rate. Simulations were performed with an upper limit of 0.5 of the membrane protein surface area occupancy. (B) The effects of simultaneously inhibiting SecA and ribosome.