Figure 2

FFRP target gene overlap and co-expression demonstrate both functional and contextual divergence in the evolution of the 8 homologous FFRPs. Nodes are FFRPs (grey triangles), extra domains for sensing effector molecules (yellow diamonds), or effector molecules (cyan circles). Purple edges indicate the preference of an FFRP for a particular effector molecule. Interaction of an FFRP with effector molecules happens through the RAM domain or extra domains (TRASH or TrkA_C) that have fused to the FFRP. Green edges indicate significant co-expression (correlation coefficient <0.5 and p-value <0.05) of two FFRPs across a panel of 35 experimental contexts. Red edges indicate significant overlap (Bonferroni corrected p-value <0.05 and percent overlap ?50%) between the experimentally determined target genes for two FFRPs. Blue edges indicate significant similarity (Bonferroni corrected p-value <0.05) between the DNA recognition motifs for two FFRPs. Below network is a scale showing that co-expression suggests functional divergence and similarity in targets and DNA recognition motifs suggests contextual divergence. Highlighted in red with a dashed red boundary is an excellent example of contextual divergence where Trh3 and Trh4 have similar target genes, DNA recognition and effector molecule preferences motifs but anti-correlated expression profiles. Highlighted in green with a dashed green border is an excellent example of functional divergence between Trh2 and VNG1179C which have similar expression profiles but different target genes, DNA recognition motifs and effector molecule preferences by fusing different extra domains.