Figure 4

AsnC acts as an activator of 158 genes under normal conditions and its regulation is turned off during PQ induced stress. A. Expression of AsnC (dashed lines) and the median expression of its 158 target genes (solid lines) for four biological replicates (black, red, orange and green) are strongly correlated when 4 mM PQ is added to the culture (PQ is added at 0 minutes and first microarray sampling is -1 minutes) [35]. Red arrows indicate the sampling times of 1 and 160 minutes used for microarray validations. B. Expression of AsnC (dashed lines) and the median expression of its 158 target genes (solid lines) are strongly correlated when 4 mM PQ is removed and the cells are allowed to recover for 4 hours (PQ is removed at 0 minutes and first microarray sampling is taken at 0 minutes) [35]. C. Distribution of correlation coefficients for all 2,400 genes on the bottom (grey), all 2,400 genes in the high PQ conditions (blue), 426 AsnC target genes in the high PQ conditions (red) and the 158 correlated AsnC target genes in the high PQ conditions (purple). Red dashed line indicates median correlation coefficient of 426 AsnC target genes in the high PQ conditions used as a cutoff to discover 158 correlated AsnC target genes. D. Relative activation is significantly lower for the 158 AsnC target genes between △ura3 △asnC and △ura3 control strain (p-value =0.05). ‘*’ means Wilcoxon rank-sum test p-value ≤0.05.