Spatio-temporal model validation workflow. Workflow comprising all steps from model construction to model validation. The first step (1) describes the construction of the model from biological observations and/or literature. In the second step (2) the model is simulated to generate time series data which is passed to the spatio-temporal analysis module for automatic detection and analysis of spatial patterns and clusters of such patterns. The third step (3) comprises the translation of the natural language specification of the system to a formal probabilistic BLSTL (PBLSTL) specification. Finally the fourth step (4) describes the validation of the model with respect to the PBLSTL specification using the model checker Mudi. In case the model is invalid it is updated and the validation procedure repeated.