Fig. 2

Transcriptome signatures of ES cell lines. Gene expression profiling of global mRNA levels from WT (n = 2), calr ^−/−(n = 5), NP (n = 3) and PC (n = 3) truncation variants were analyzed by agglomerative unsupervised clustering analysis, with mean expression of genes in each condition organized into a heatmap dendrogram. Data represents an update of GEO dataset (GDS3729). WT and calr ^−/− together were distinct from NP and PC profiles, with a calculated Euclidean distance metric of 0.655 (Branch I). Distance metrics between WT and calr ^−/− and between NP and PC variants indicate a similar degree of distinction at Branch II. Colorscale indicates normalized intensity values as a metric of expression for up and down regulated genes in red and green, respectively