Fig. 4

Deconvolution of cardiopathologic gene ontology remodeling discretely prioritized by CALR^−/−-PC. Refinement of quality controlled gene lists allowed gene ontology analysis for identification of prioritized functional themes. a Volcano plot analysis yields up and down regulated genes that satisfy significance and fold change criteria that report specific gene ontology categories upon pathway analysis. Shown is the gene expression distribution within the quality controlled transcriptome of the PC truncation variant. Horizontal green line indicates P-value cutoff (P = 0.05). Vertical green lines delimit 2.0x fold change threshold. Orange indicates genes that meet or exceed p-value and fold change criteria, while gray represents genes that fall below significance and fold change limits. b Enriched functional themes are encoded within a finite number of up and down regulated transcripts. In the PC variant, a larger proportion of genes are up regulated. c As the down regulated component of the PC transcriptome harbored over representation of the Cardiovascular Disease functional category, top pathways in the down regulated component of the PC transcriptome were examined to determine identities of enriched signaling cascades. Here, EIF2 signaling was highly prioritized. Pathway nomenclature is shown on the x-axis of the histogram, while –log (p-value) is provided on the y-axis. Inset – Investigation of EIF2 signaling revealed significant down regulated expression of 15 genes, of which RPL32 was the most significantly decreased. Shown are -log₁₀ (p-value) on the x-axis, and absolute fold change on the y-axis