Fig. 1

The pathway topology proposed in [25]. We used this model as our true model and generated data based upon it. The black lines with small arrow tips depict the transition between different species in the model like production, degradation or complex formation. The black lines with open circle tips depict the phosphorylation process by kinases. The lines with open triangle tips show activating regulatory interactions whereas lines with blunt ends show deactivating regulatory interactions. The red colored double arrow denotes the post translational regulation of glycerol production by the active phosphorylated Hog1 protein. We did not consider this regulatory interaction in our simplified model. The dotted ellipses in the upper left hand corner indicate the two different upstream activation routes important in our study. Parts of the pathway whose parameters were affected by the choice of the partitioning scheme were highlighted yellow and gray. We explained the changes in the parameters of those regions in our results section. (Figure adopted from [23])