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Fig. 2 | BMC Systems Biology

Fig. 2

From: Complex regulation of Hsf1-Skn7 activities by the catalytic subunits of PKA in Saccharomyces cerevisiae: experimental and computational evidences

Fig. 2

De-repression of HSE-dependent gene expression in cdc25Δ cells is dependent on both Hsf1 and Skn7 activities. Strains transformed with reporter plasmid pRY016 (2 μ) were grown in SD medium at 25 °C until mid-exponential phase and treated at different temperatures as described in Methods section. Values are reported as β-galactosidase specific activity (nmol of hydrolyzed ONPG min−1 mg−1 protein) and are the average and standard deviation of at least three independent experiments. Bars that do not share at least a common letter differ significantly (P < 0.05). Strains assayed were: a WT (W303-6B), hsf1-ΔCTA (LM020), cdc25Δ (SL5001), and hsf1-ΔCTA cdc25Δ (SL6001). b WT (W303-6B), skn7Δ (SE1000), cdc25Δ (SL5001), and cdc25Δ skn7Δ (SL4001)

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BMC Systems Biology

ISSN: 1752-0509

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