Fig. 7

Comparison of the fluxes of 19 central metabolic reactions of the R. jostii RHA1 network a estimated using ¹³C-MFA for non-limited growth phase and using FBA for N-limited phase with glucose b or acetate c as substrate. For the non-limited phases, fluxes were calculated using the ¹³C-MFA approach. The data points (circle) reported are the average of 100 solutions inversely weighted on the fitting error of the ¹³C-MFA model; the error bars are Cochran’s weighted standard errors [48]. For the N-limited phases, the ranges of fluxes (shadow between maximum and minimum) were computed using the genome-scale FBA at zero nitrogen uptake rate. Note that the y-axis scales for panels b and c are not the same, and that the y-axis of non-limited data and N-limited ranges of fluxes are not the same in panel c. The x-axis includes the reaction IDs appeared in the simplified central metabolic network of R. jostii RHA (panel a). The corresponding KEGG IDs can be found with the complete central metabolic network in Additional file 6. The reactions in parentheses are the backward reactions of the reversible reactions