Table 1 Features of different enzyme kinetic parameter estimation methods. “WLS” stands for the weighted least squares method. “TLS” stands for the total least squares method. “Raaijmakers” is the maximum likelihood method of Raaijmakers
Multiple substrates/ | Reversible | Relative | Error in all | |
|---|---|---|---|---|
products | reaction | error | variables | |
Double reciprocal | ✗ | ✗ | ✗ | ✗ |
Direct linear | ✗ | ✗ | ✓ | ✓ |
WLS | ✓ | ✓ | ✗ | ✗ |
TLS | ✓ | ✓ | ✗ | ✓ |
Raaijmakers | ✗ | ✗ | ✓ | ✗ |
InVEst | ✓ | ✓ | ✓ | ✓ |