Fig. 7

Application scenario I Pareto Front in blue line connected dots. A. Dots with reddish color are obtained when using the RBS strength of gene C as a trade-off tuning knob and represented by modifying k p _C ∈ [ 5 0.05] starting at the extreme solution. Notice, that decreasing only k p _C it is possible to increase the sensitivity, almost without losing optimality (without getting away from the Pareto front). Inset shows the time course of protein C. As expected, sensitivity of the solution is increased, i.e. the peak of protein concentration after stimulus is higher. B. Dots with blueish color are obtained when using the promoter strength and plasmid copy number gene B by modifying K m _B C g B∈ [ 200 1]