Table 1 E. coli strains, plasmids, and primers used in this study

B

Wild type

ATCC11303

SZ420

E. coli B, ΔfrdBC ΔldhA ΔackA Δ(focA-pflB) ΔpdhR::pflBp ₍₆₎-(aceEF-lpd)

Zhou et al. [28]

AI03

E. coli SZ420, ΔadhE

Iverson et al. [13]

AI05

E. coli SZ420, ΔadhE ΔptsG

Iverson et al. [13]

AI09

E. coli SZ420, ΔadhE ΔptsG ΔxylB

This study

AI12

E. coli SZ420, ΔadhE ΔptsG ΔxylB ΔsdhCp::Fnr box- pflBp ₍₆₎-(sdhCDBA-sucABCD)

This study

AI21

E. coli SZ420, ΔadhE ΔptsG ΔxylB ΔxylA ΔsdhCp::Fnr box- pflBp ₍₆₎-(sdhCDBA-sucABCD)

This study

Plasmids

pKD4

bla, FRT-km-FRT

Datsenko and Wanner [10]

pKD46

bla, γ β exo (red recombinase), temperature-conditional replicon

Datsenko and Wanner [10]

pFT-A

bla, flp, temperature-conditional replicon

Posfai et al. [19]

pUC19

bla cloning vector

NE Biolab

pSD105

PCR amplified 0.35 kb pflB promoter region (BamHI- pflBp ₆-HindIII) was inserted into pSD101 at BamHI and HindIII sites

Zhou et al. [29]

pAGI02

PCR amplified 0.966 kb xylI region from C. boidinii was inserted into pSD105 at HindIII site

Iverson et al. [13]

Primers^a

ΔxylB N-primer

atgtatatcgggatagatcttggcacctcgggcgtaaaagttattgtgtaggctggagatgcttc

This study

ΔxylB C-primer

ttacgccattaatggcagaagttgctgatagaggcgacggaacgtcatatgatatcctccttag

This study

ΔxylA N-primer

ccgcggcattacctgattatggagttcaatatgcaagcctattttggtgtaggctggagatgcttc

This study

ΔxylA C-primer

gttatttgtcgaacagataatggtttaccagattttccagttgttccatatgaatatcctccttag

This study

Integration primer 1

ccgacaaactatatgtaggttaattgtaatgattttgtgaacagcctatactgccgccag gtgtaggctggagctgcttc (used as N-terminal primer for amplifying FRT-kan-FRT-Fnr box- pflBp ₍₆₎-sdhC’)

This study

Integration primer 2

gaaccggatggtctgtaggtccagattaacaggtctttgttttttcacatttcttatcat gtaacacctaccttctgttgctgtgatatagaagac (used as C-terminal primer for amplifying FRT-kan-FRT- pflBp ₆-sdhC’)

This study

rrsA primer 1

cggtggagcatgtggtttaa (used for qt-PCR)

Nishino et al. [18]

rrsA primer 2

gaaaacttccgtggatgtcaaga(used for qt-PCR)

Nishino et al. [18]

sdhC primer 1

cgccagccgcccagcacag (used for qt-PCR)

This study

sdhC primer 2

ggtatggaaggtctgttccgtcagattggtatttacagccc (used for qt-PCR)

This study

sucA primer 1

cagggcggttgcttcaccatctcca (used for qt-PCR)

This study

sucA primer 2

gcggcacgaactctttaccattccacacc (used for qt-PCR)

This study