Fig. 9
Increasing C dimer binding cooperativity in wt EcoRV. The binding cooperativity ω is increased from the absence of cooperativity (ω = 1, corresponding to wt EcoRV), to the higher values corresponding to cooperative C dimer binding. For each curve, ω is increased in steps by a factor of 2, and the effect is assessed on: a The dynamics of R and M synthesis. The solid and the dashed curves correspond, respectively, to the dynamics of R and M synthesis. The black curve corresponds to wt (no cooperativity), with the curves fading, as the cooperativity increases (with the light gray corresponding to maximal ω). b The transition velocity vmax. c The stability of R steady-state levels