Fig. 3

Topological parameter profiles of NaV1.7 gain-of-function mutations and nABN and hSNPs. a The upper panel shows the B _ct profile of gain-of-function mutation; the lower panel show the B _ct profile of nABN and hSNPs. Squares indicates B _ct values of WT amino acids, circles indicate B _ct values of mutated amino acids. The graphs highlight that the difference between B _ct value of mutated amino acids and B _ct value of WT amino acids is higher in the cohort of gain-of-function (GF) mutations (upper panel) compared to control (Ctrl) nABN and hSNPs (lower panel). B _ct values are multipled by 100. b The box plot shows the |ΔB _ct | difference between gain-of-function mutations and the cohort of nABN and hSNP variants (mean gain-of-function |∆B _ct | = 1.14 ± 1.40; nABN and hSNP ∆B _ct  = 0.19 ± 0.28; ***p < 0.001 by Wilcoxon signed-ranked test). |∆B _ct | values are multipled by 100; dark horizontal lines and the triangular symbol represent median and mean values respectively, with the box representing the 25th and 75th percentiles, the whiskers the 5th and 95th percentiles, and the dots the outliers. c The upper panel shows the Degree (D) profile of gain-of-function mutations; the lower panel show the D profile of nABN and hSNPs. Squares indicates D values of WT amino acids, circles indicate D values of mutated amino acids. The box plot shows the |ΔD| difference between gain-of-function (GF) mutations and the cohort of nABN and hSNP (Ctrl) variants (gain-of-function mean |∆D| = 4.3 ± 5.15; nABN and hSNP |∆D| = 2.37 ± 2.10; p > 0.05 by Wilcoxon signed-ranked test); dark horizontal lines and the triangular symbol represent median and mean values respectively, with the box representing the 25th and 75th percentiles, the whiskers the 5th and 95th percentiles, and the dots the outliers. d The upper panel shows the Clustering Coefficient (CC) profile of gain-of-function mutations; the lower panel show the CC profile of nABN and hSNPs. Squares indicates CC values of WT amino acids; circles indicate CC values of mutated amino acids. The box plot shows the |ΔCC _ct | difference between gain-of-function (GF) mutations and the cohort of nABN and hSNP (Ctrl) variants (mean gain-of-function |∆CC _ct | = 0.15 ± 0.20; nABN and hSNP |∆CC _ct | = 0.20 ± 0.25; p > 0.05 by Wilcoxon signed-ranked test); dark horizontal lines and the triangular symbol represent median and mean values respectively, with the box representing the 25th and 75th percentiles, the whiskers the 5th and 95th percentiles, and the dots the outliers. e The upper panel shows the Closeness (C_ct) profile of gain-of-function mutations; the lower panel show the C_ct profile of nABN and hSNPs. Squares indicates C_ct values of WT amino acids, circles indicate C_ct values of mutated amino acids. The box plot shows the ΔC _ct difference between gain-of-function (GF) mutations and the cohort of nABN and hSNP (Ctrl) variants (mean gain-of-function |∆C _ct | = 0.6 ± 0.9; nABN and hSNP |∆C _ct | = 0.7 ± 1.4; p > 0.05 by Wilcoxon signed-ranked test). C _ct and ∆C _ct values are multipled by 100; dark horizontal lines and the triangular symbol represent median and mean values respectively, with the box representing the 25th and 75th percentiles, the whiskers the 5th and 95th percentiles, and the dots the outliers. f The upper panel shows the Eccentricity (E_ct) profile of gain-of-function mutations; the lower panel show the E_ct profile of nABN and hSNPs. Squares indicates E_ct values of WT amino acids, circles indicate E_ct values of mutated amino acids. The box plot shows the |ΔE _ct | difference between gain-of-function (GF) mutations and the cohort of nABN and hSNP (Ctrl) variants (mean gain-of-function |∆E _ct | = 1.53 ± 3.75; nABN and hSNP |∆E _ct | = 2.05 ± 4.62; p > 0.05 by Wilcoxon signed-ranked test); dark horizontal lines and the triangular symbol represent median and mean values respectively, with the box representing the 25th and 75th percentiles, the whiskers the 5th and 95th percentiles, and the dots the outliers