Fig. 1

Workflow of dynamic proteomics in response to Hsp90 inhibitor drug: We used the LARC library of over 1000 H1299 cancer cell clones; in each clone a protein is tagged fluorescently at its natural locus using exon tagging. We selected 100 proteins in diverse pathways and functions, and assayed their dynamics in the presence of the drug, as well as in a control conditions. Time lapse movies over 20 h were conducted in 96-well format. Automated image analysis captured the protein dynamics at the individual cell level, as well as mitosis and death events for each cell