Fig. 2

The kinetics of model variables during batch growth of normal, non-FA producing lines of E. coli cells. a. The blue line and symbols show a growth curve (OD units) in the model and published data [6]. Three phases of growth are indicated by black vertical lines: 1-exp. phase, 2-exp.-to-stat transition phase, 3-stat phase. ppGpp concentrations in the model and published data [6] are shown by the red line and symbols respectively. The end of phase 1 coincides with an increase of ppGpp concentration and the depletion of limiting nutrient lim (green dotted line). The end of phase 2 coincides with the depletion of the growth-supporting nutrient nutr (green solid line). The transcriptional activity of P1/P2 promoter (normalized to maximum) is shown by black solid line and black symbols for the model and the data [6] respectively. The relative ribosomes number is shown by the orange line. The data on ppGpp kinetics were quantified based on available measurements of ppGpp concentrations [8]. b. The kinetics of Acyl-ACP, ppGpp and FAS/PLS flux are shown by black, red and blue colours respectively