Table 1 Overexpression targets simulated by tSOT to increase DCAs production
From: Genome-scale model-driven strain design for dicarboxylic acid production in Yarrowia lipolytica
Targets | Reaction Name | Reaction Definition | Yield Improvement (%) |
|---|---|---|---|
GLUDy | Glutamate Dehydrogenase (NADPH-forming) | glu_L[c] + h2o[c] + nadp[c] < => akg[c] + h[c] + nadph[c] + nh4[c] | 22.2 |
MDH | Malate Dehydrogenase (cytosol) | mal_L[c] + nad[c] < => h[c] + nadh[c] + oaa[c] | 47.8 |
MDHm | Malate Dehydrogenase (mitochondrial) | mal_L[m] + nad[m] < => h[m] + nadh[m] + oaa[m] | 47.8 |
ME1m | Malic enzyme (NAD-dependent) | mal_L[m] + nad[m] - > co2[m] + nadh[m] + pyr[m] | 47.8 |