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Table 1 Strain details from growth characterizations

From: Reframing gene essentiality in terms of adaptive flexibility

Keio strain Possible alternate Final cell density (gDW/L) Time to > half Mutations
  genes/pathways *mean, Std. Dev., %RSD final density (Hrs) flask1
    *mean, Std. Dev., %RSD population?
WT glucose - 1.0, <0.01, 0.2% 10, <1, <0.01% No
WT glycerol - 1.1, 0.04, 3% 12, <1, 0.04% No
Δ ubiE Alternate growth using 0.43, 0.08, 20% 14, 1, 9% No
  demethylmenaquinone1    
Δ cysK cysM 2 1.0, 0.1, 10% 21, <1, <0.01% Variable
Δ metL (thrA or lysC)2 1.0, 0.1, 9% 28, 2, 7% Variable
Δ metC (tnaA or malY)2, 0.28, 0.1, 50% 36, 9, 20% Variable
  (malY, alr, fimE)3    
Δ thrA (metL or lysC)2 1.1, 0.1, 8% 43, <1, 0.04% Yes
Δ carA (yahI or arcC or yqeA)2, 0.50, 0.2, 40% 58, 17, 30% Variable
  (carB, ygiT, cho, yncK)3    
Δ cysP (modA + modB + modC)2 1.1, 0.1, 10% 65, <1, 0.2% No
Δ proA argE 2 1.1, 0.3, 20% 190, 26, 10% Yes
Δ proB argE 2 0.66, 0.3, 50% 83, 10, 10% Yes
Δ ptsI galP2, (fucP, xylE, galE)3 1.1, 0.1, 10% 100, 5, 5% Yes
Δ serB glyA2, (gph, hisB, ytjC)3 0.95, 0.2, 20% 110, 8, 7% Yes
  1. 1Evidence for this described in [64]. 2In silico prediction, iJO1366 genome-scale model of metabolism [13]. 3Experimental multicopy suppression evidence [19]. *The data used from triplicate experiments represented in Fig. 1b was used to calculate means, standard deviations (St. Dev.), and percent relative standard deviations (%RSD)