Table 1 Strain details from growth characterizations
From: Reframing gene essentiality in terms of adaptive flexibility
Keio strain | Possible alternate | Final cell density (gDW/L) | Time to > half | Mutations |
|---|---|---|---|---|
| Â | genes/pathways | *mean, Std. Dev., %RSD | final density (Hrs) | flask1 |
| Â | Â | Â | *mean, Std. Dev., %RSD | population? |
WT glucose | - | 1.0, <0.01, 0.2% | 10, <1, <0.01% | No |
WT glycerol | - | 1.1, 0.04, 3% | 12, <1, 0.04% | No |
Δ ubiE | Alternate growth using | 0.43, 0.08, 20% | 14, 1, 9% | No |
| Â | demethylmenaquinone1 | Â | Â | Â |
Δ cysK | cysM 2 | 1.0, 0.1, 10% | 21, <1, <0.01% | Variable |
Δ metL | (thrA or lysC)2 | 1.0, 0.1, 9% | 28, 2, 7% | Variable |
Δ metC | (tnaA or malY)2, | 0.28, 0.1, 50% | 36, 9, 20% | Variable |
| Â | (malY, alr, fimE)3 | Â | Â | Â |
Δ thrA | (metL or lysC)2 | 1.1, 0.1, 8% | 43, <1, 0.04% | Yes |
Δ carA | (yahI or arcC or yqeA)2, | 0.50, 0.2, 40% | 58, 17, 30% | Variable |
| Â | (carB, ygiT, cho, yncK)3 | Â | Â | Â |
Δ cysP | (modA + modB + modC)2 | 1.1, 0.1, 10% | 65, <1, 0.2% | No |
Δ proA | argE 2 | 1.1, 0.3, 20% | 190, 26, 10% | Yes |
Δ proB | argE 2 | 0.66, 0.3, 50% | 83, 10, 10% | Yes |
Δ ptsI | galP2, (fucP, xylE, galE)3 | 1.1, 0.1, 10% | 100, 5, 5% | Yes |
Δ serB | glyA2, (gph, hisB, ytjC)3 | 0.95, 0.2, 20% | 110, 8, 7% | Yes |