Workflow and characteristics of context-specific models. (A) Two context-specific multi-tissue metabolic networks were built using post-absorptive exchange constraints, gene expression data, the GIMME algorithm, and flux variability analysis. The two models detailed the metabolism of obese and type II diabetes obese individuals based on the prolonged starvation multi-tissue simulation. (B) We compared the reaction activity of the two context-specific models. First, we looked at how reactions were expressed based solely on the gene expression data (left column). Second, we looked at reaction activity by determining the flux variability of the two context-specific models from the expression data and GIMME algorithm (right column).