A quantitative model of the initiation of DNA replication in Saccharomyces cerevisiae predicts the effects of system perturbations

Table 1 Kinetic reaction rates describing the network

Rate	Description	Rate equation
	Expression & Degradation
v₁	Expression of CDC6	k₁
v₂	Degradation of CDC6	k₂CLB5·CDC6
v₃	Expression of DBF4	k₃
v₄	Degradation of DBF4	k₄DBF4·CDC20
	Formation of the Pre-Replicative Complex
v₅	Association of ORC and CDC6	k₅RC1·CDC6 – k_5rRC2
v₆	Association and nuclear import of MCM and CDT1	k₆MCM_C·CDT1_C/(KM₁ + MCM_C)
v₇	Loading of MCM by CDT1	k₇RC2·MCM·CDT1
v₈	Nuclear export of CDT1	k₈CLB5·CDT1
v₉	Dissociation of nuclear MCM-CDT1 complex	k₉MCM·CDT1 – k_9rMCM·CDT1
v₁₀	Dissociation of CDC6 from the Pre-RC	k₁₀RC3 – k_10rCDC6·RC4
	Formation of the Pre-Initiation Complex
v₁₁	Dissociation of ORC and MCM from Pre-RC	k₁₁RC4
v₁₂	Association of DBF4 and the Pre-RC	k₁₂RC4·DBF4 – k_12rRC5
v₁₃	Association of CDC45 and the Pre-RC	k₁₃RC5·CDC45·CLB5/(KM₂ + CDC45)
	Post-Replicative Complex and Ensuing Events
v₁₄	Origin firing	k₁₄RC6
v₁₅	Breakup of the elongation fork	k₁₅FORK
v₁₆	Nuclear export of MCM	k₁₆MCM·CLB5
v₁₇	Phosphorylation of ORC	k₁₇RC7/(1 + (CLB5/k₁₈)⁵)

ISSN: 1752-0509