Skip to main content

Table 2 Mass isotopomer distribution of lactate, glycogen and ribose after incubations with resistin and/or insulin

From: 13C metabolic flux analysis shows that resistin impairs the metabolic response to insulin in L6E9 myotubes

  Mass isotopomer distributions (%)
Incubation condition
Ins- Ins+
Res- Res+ Res- Res+
Lactate     
m0 88.56±0.98 88.74±0.25 87.68±0.22 87.63±0.17
m1 0.70±0.34 0.48±0.11 0.63±0.06 0.52±0.14
m2 10.53±0.30 10.85±0.15 11.69±0.05* 11.78±0.11
m3 0.21±0.63 0.07±0.18 0.00±0.11 0.07±0.13
Glycogen     
m0 67.28±0.65 69.20±1.41 65.14±1.08 64.48±0.45
m1 0.29±0.03 0.36±0.14 0.96±0.10 0.84±0.10
m2 31.78±0.74 29.91±1.02 33.20±1.18 33.78±0.76
m3 0.53±0.19 0.43±0.28 0.43±0.07 0.60±0.20
m4 0.00±0.02 0.00±0.06 0.17±0.05 0.15±0.02
Ribose     
m0 96.13±0.55 94.28±0.76 94.47±0.48 92.83±1.18
m1 1.34±0.23 1.45±0.11 2.56±0.14 2.97±0.15
m2 0.68±0.19 1.14±0.16* 1.18±0.09* 1.40±0.23
m3 1.17±0.63 1.56±0.58 0.87±0.17 1.14±0.32
m4 0.50±0.44 1.17±0.22* 0.61±0.14 1.17±0.41
  1. L6E9 myotubes were treated (Res+) or not treated (Res-) with 100 nM resistin for 8h and then incubated for 6 h with 10 mM glucose, 50%-enriched in [1,2-13C2]-glucose in the absence (Ins-) or presence (Ins+) of 100 nM insulin. Mass isotopomer distributions at the end of incubations were determined, as described in the Methods section. Results are the mean±standard deviation (n = 3). (*) indicates p<0.05 compared with the condition of neither resistin nor insulin.