13C metabolic flux analysis shows that resistin impairs the metabolic response to insulin in L6E9 myotubes

BMC Systems Biology

Table 2 Mass isotopomer distribution of lactate, glycogen and ribose after incubations with resistin and/or insulin

	Mass isotopomer distributions (%)
	Incubation condition
	Ins-		Ins+
	Res-	Res+	Res-	Res+
*Lactate*
m0	88.56±0.98	88.74±0.25	87.68±0.22	87.63±0.17
m1	0.70±0.34	0.48±0.11	0.63±0.06	0.52±0.14
m2	10.53±0.30	10.85±0.15	11.69±0.05*	11.78±0.11
m3	0.21±0.63	0.07±0.18	0.00±0.11	0.07±0.13
*Glycogen*
m0	67.28±0.65	69.20±1.41	65.14±1.08	64.48±0.45
m1	0.29±0.03	0.36±0.14	0.96±0.10	0.84±0.10
m2	31.78±0.74	29.91±1.02	33.20±1.18	33.78±0.76
m3	0.53±0.19	0.43±0.28	0.43±0.07	0.60±0.20
m4	0.00±0.02	0.00±0.06	0.17±0.05	0.15±0.02
*Ribose*
m0	96.13±0.55	94.28±0.76	94.47±0.48	92.83±1.18
m1	1.34±0.23	1.45±0.11	2.56±0.14	2.97±0.15
m2	0.68±0.19	1.14±0.16*	1.18±0.09*	1.40±0.23
m3	1.17±0.63	1.56±0.58	0.87±0.17	1.14±0.32
m4	0.50±0.44	1.17±0.22*	0.61±0.14	1.17±0.41

L6E9 myotubes were treated (Res+) or not treated (Res-) with 100 nM resistin for 8h and then incubated for 6 h with 10 mM glucose, 50%-enriched in [1,2-¹³C₂]-glucose in the absence (Ins-) or presence (Ins+) of 100 nM insulin. Mass isotopomer distributions at the end of incubations were determined, as described in the Methods section. Results are the mean±standard deviation (n = 3). (*) indicates p<0.05 compared with the condition of neither resistin nor insulin.

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