Figure 4

Calibration of model A to experimental data. (A) Endogenous concentrations of PKD, PI4KIII β and CERT from the absolute quantification experiments are depicted together with the model fit of the steady state distributions. (B) Model fit of ectopic expression of PI4KIII β with subsequent activation of PKD via PDBu at 24 h. Depicted are the the rise of the overall PI4KIII β level and the change of PKD and PI4KIII β phosphorylation after PDBu addition. (C) Model fit of ectopic expression of CERT with subsequent inhibition of PKD via kb-NB142-70 after 24 h. Shown are the the rise of the overall CERT level and the change of PKD and CERT phosphorylation after kb-NB142-70 addition. (D) Refinement experiments focusing on measurements of early PKD phosphorylation during ectopic expression of PI4KIII β and long time change in PKD phosphorylation up to 6 hours after PDBu addition. (E) Validation experiment. PI4KIII β phosphorylation was measured at 25.25 h after ectopic expression of PI4KIII β and inhibition of PKD via kb-NB142-70 at 24 h. Data were not used for model calibration.