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Table 6 Principal experimental methods for GE quantification

From: Integrating genetics and epigenetics in breast cancer: biological insights, experimental, computational methods and therapeutic potential

Method Pros Cons
Northern Blotting -Inexpensive -low throughput
-detecting transcript size -semiquantitative
-RNAase contamination
RT-PCR -high sensitivity -high variability
-high sequence specific -normalizaton methods
Microarray -measurement of the activity of thousands of genes at once -high cost
-rapid -analysis of Big data
-don't require large-scale DNA sequencing -high Background noise
Sanger sequencing technology -low Background noise -only a portion of the transcript
-isoforms are generally indistinguishable from each other
-Low throughput
RNA-seq -measurement of the activity of thousands of genes at once -High cost
-require low amount of RNA -Analysis of Big data
-high reproducibility
-Low Background noise