Fig. 5

The duration of the first seeding regulates MSC fates via mechanical memory. The first seeding stiffness in this figure is 30 kPa. The second seeding stiffness is 0.4 kPa (a), 0.9 kPa (b) or 12 kPa (c). When the duration of the first seeding is 50 (blue lines), MSCs undergo osteogenic differentiation according to memory. When the duration of the first seeding is 15 (red lines), MSCs undergo myogenic differentiation. When the duration of the first seeding is 5 (brown lines in columns A and B), MSCs differentiate into adipocytes or myogenic cells. When the duration of the first seeding is 0.5 (pink lines in column A), MSCs are able to undergo adipogenic, myogenic, or neurogenic differentiation. Finally, when the duration of the first seeding is 0 (black lines), MSCs are able to undergo adipogenic, myogenic, or neurogenic differentiation