Fig. 8

Bicoid, Huncback, and Dicheate are essential for expression driven by s250. For each sequence, the binding structure structure is shown (left). Height of bars is proportional to LLR of binding for each motif. A subset of motifs are shown. Binding sites for all factors considered in this work are included in Additional file 1: Figures S5-S15. We also show FISH for lacZ driven by each of the sequences (right). a s250. The the factor Dicheate has been included in the binding structure in grey. The enhancer drives strong expression. b s250 with Bcd and Hb orientation reverted to the orientation present in s272. The resulting enhancer does not drive expression. c s250 with the a single Dicheate site removed. The resulting enhancer does not drive expression