MicroRNA sequence and expression database
© Kaya et al; licensee BioMed Central Ltd. 2007
Published: 8 May 2007
Data and methods
Bilkent University miRNA sequence and expression database was constructed using Mysql version 14.7 on Suse Linux 10.0 server; the web interface implemented in HTML 4.0 combined with PHP version 4.4.0. Statistical calculations were performed using R package version 2.2.1. miRNA mature sequences from Homo sapiens, Mus musculus, Danio rerio and Caenorhabditis elegans were downloaded from miRBase of Sanger Institute database http://microrna.sanger.ac.uk/ version 8.2. Two independent microarray data sets [3, 4] were associated with human and mouse miRNA dinucleotide motif frequency, respectively. Human miRNA targets were extracted from Argonaute Database of Heildelberg http://www.ma.uni-heidelberg.de/apps/zmf/argonaute/interface/. GO ontology data were linked with Argaonaute gene symbols and alias data from Entrez Gene Database http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=gene.
Results and conclusions
High expression of hsa-mir-15a and hsa-mir-16, known to be deleted in chronic lymphocytic leukemia, was detected in bone marrow and spleen (Figure 2a). Analysis of these miRNAs in terms of their targets resulted in significant representation of antiapoptosis and humoral immune response GO terms, both attributable to BCL-2 (Figure 2b). Although BCL2 is well known for its role in cell survival, there is no known direct relation of BCL-2 with immune response. Future studies involve integration of multiple species-specific gene expression data sets and implementation of correspondence analysis tools for multivariate analysis of sequence and expression data. The presented database will help understand miRNAs in a systems biology context via integration of the sequence, expression, and functional attributes.
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