- Open Access
Prioritization of disease microRNAs through a human phenome-microRNAome network
- Qinghua Jiang†1,
- Yangyang Hao†1,
- Guohua Wang1,
- Liran Juan1,
- Tianjiao Zhang1,
- Mingxiang Teng1,
- Yunlong Liu2 and
- Yadong Wang1Email author
© Wang et al; licensee BioMed Central Ltd. 2010
- Published: 28 May 2010
The identification of disease-related microRNAs is vital for understanding the pathogenesis of diseases at the molecular level, and is critical for designing specific molecular tools for diagnosis, treatment and prevention. Experimental identification of disease-related microRNAs poses considerable difficulties. Computational analysis of microRNA-disease associations is an important complementary means for prioritizing microRNAs for further experimental examination.
Herein, we devised a computational model to infer potential microRNA-disease associations by prioritizing the entire human microRNAome for diseases of interest. We tested the model on 270 known experimentally verified microRNA-disease associations and achieved an area under the ROC curve of 75.80%. Moreover, we demonstrated that the model is applicable to diseases with which no known microRNAs are associated. The microRNAome-wide prioritization of microRNAs for 1,599 disease phenotypes is publicly released to facilitate future identification of disease-related microRNAs.
We presented a network-based approach that can infer potential microRNA-disease associations and drive testable hypotheses for the experimental efforts to identify the roles of microRNAs in human diseases.
- Area Under Curve
- Similar Disease
- Target Prediction Algorithm
- microRNA Family
- Disease Pair
The identification of genes associated with human diseases is an important goal of biomedical research. Recently, a number of computational methods have been developed to predict or prioritize disease-related protein-coding genes [1–23]. Most approaches are based on the idea that dysfunctions of functionally related protein-coding genes tend to be associated with phenotypically similar diseases [1, 2, 6, 12, 13, 15, 16, 18–22]. These protein-coding genes linked to similar diseases usually interact with each other or participate in the common biological modules. Network-based approaches have also been employed to predict or prioritize new candidate disease genes based upon network linkages with known disease genes [1–3, 12, 23]. These approaches typically start with constructing a gene-gene association network based on one or more types of genomic and proteomic information, and then prioritize candidate protein-coding genes based on network proximity to known disease-related genes. For example, Franke et al. and Linghu et al. separately constructed a functional linkage network (FLN) by integrating multiple types of data, such as protein-protein interaction, microarray and Gene Ontology annotation data, and utilized the FLN for disease gene prioritization [7, 23]. Lage et al. constructed a human phenome-interactome network and scored each candidate protein based on the involvement of its direct network neighbors in similar diseases . The biological interpretation of a high-scoring candidate was that the candidate was likely to be involved in the molecular mechanism of the disorder of interest.
Growing evidence indicates that microRNAs play important roles in the development and progression of human diseases, and are critical for the prognosis, diagnosis and the evaluation of treatment responses for these diseases [24–37]. Jiang et al. and Lu et al. independently developed two manually curated database--miR2Disease  and Human MicroRNA Disease Database (HMDD) , which aim at providing a comprehensive resource of experimentally verified microRNA-disease associations. However, one major issue in microRNA studies is the lack of bioinformatics methods to infer potential microRNA-disease associations that can guide further biological experiments.
MicroRNAs exert their biological functions through suppression of their target genes . Accumulating studies indicate that microRNAs usually perform related functions by targeting either the same genes or functionally related genes in a coordinated manner [34, 35, 41–48]. It has become an increasingly important and informative approach to analyze biological systems and disease mechanisms in networks of genes and diseases [6, 12, 21, 49, 50]. Establishing a functional relationship between two microRNAs by their target genes and further constructing a functionally related microRNA network will be useful for understanding the roles of microRNAs in diseased states.
Construction of human phenome-microRNAome network
In order to prioritize the entire microRNAome for diseases, we constructed a functionally related microRNA network by assuming that two microRNAs are functionally related if the overlap between their target genes was statistically significant (Figure 1A). A p-value from Fisher’s Exact Test was used to evaluate the overlap, and was adjusted by the Benjamini-Hochberg correction [51, 52]. Two microRNAs were considered to be functionally related if the adjusted p-value was less than 0.001. Following this strategy, and using microRNA-target dataset predicted by Probability of Interaction by Target Accessibility (PITA) , we obtained a functionally related microRNA network that included 9,249 relationships (edges) between 514 microRNAs (nodes). We subsequently constructed a hypothetical human phenome-microRNAome network by integrating the microRNA network with a phenome network [1, 12, 20] using 270 experimentally verified microRNA-disease associations (Figure 1B).
Functionally related microRNAs tend to be associated with phenotypically similar diseases
Our model was based on the notion that functionally related microRNAs tend to be associated with phenotypically similar diseases. We examined it by addressing two questions: (1) whether disease pairs associated with common microRNAs are phenotypically more similar, as opposed to randomly selected phenotype pairs; and (2) whether the microRNA pairs associated with common diseases are functionally more related. Because we have constructed a functionally related microRNA network, the functional relatedness between two microRNAs can be measured through the number of shared network neighbors and the length of the shortest path in the microRNA network. We chose to use these two measures mainly based on the standpoint that, in a functional network, if two nodes are less distant from each other or share more neighbors, they are functionally more related. Herein, we used the function e -x to convert the length of the shortest path to the degree of functional relatedness between two microRNAs.
In order to assess the power of our model to infer microRNA-disease associations by prioritizing the entire microRNAome, we performed the leave-one-out cross-validation on 270 known experimentally verified microRNA-disease associations. Each association was left out once as the testing case, being referred to as <m, d>. For the disease d, the microRNA m was termed ‘defector’ microRNA. We prioritized the entire microRNAome according to the scores derived from the scoring system. Note that the score can be computed only for all microRNAs in the microRNA network, which was termed the ranked microRNAome. If the ranking of the ‘defector’ microRNA exceeds a given threshold, the model successfully predicts the experimentally verified association <m, d>.
In order to ensure that the prioritization represents biological significance, the model was tested on the 270 randomly generated microRNA-disease associations, which resulted in an AUC of 49.81% (green curve in Figure 3), approximate to the uninformative AUC of 50% (dash line in Figure 3). This result showed indirectly that our model can obtain a biologically meaningful prioritization.
Applicability of the model to diseases without any known related microRNAs
To demonstrate that our model is applicable to the diseases without any known related microRNAs, we removed all other experimentally verified microRNA-disease associations that are involved in the disease d, for each of the 270 known experimentally verified microRNA-disease associations, denoted as <m, d>. This step ensured that prioritizing microRNAs for the disease d only took advantage of the information of other diseases having similar phenotypes with the disease d. When our model was tested on this dataset, an AUC of 69.51% was obtained (blue curve in Figure 3), suggesting that the model had the potential to achieve the goal of predicting potential microRNA-disease associations for the diseases without any known related microRNAs.
Effect of microRNA families and robustness
MicroRNAs belonging to the same family have similar target profiles because they share the “seed” region close to the 5’ end of the microRNAs, which is the main determinant of microRNA targeting. One possible concern is the potential confounding effect of microRNA families in the performance evaluation procedure. If several microRNAs (m i , i=1, 2…) belonging to the same family are associated with a certain disease d, it might be relatively easy for the leave-one-out cross-validation procedure to recover the experimentally verified microRNA-disease association <m i, d> being examined. To assess the possible effect of this confounding factor, we removed all other experimentally verified associations between the disease d and microRNAs which belong to the same family as the microRNA m. Following this procedure, a slightly reduced AUC of 71.39% was achieved (black curve in Figure 3), suggesting that microRNA families are not a main factor leading to the good performance of our model.
There is great difference among the microRNA-target lists predicted by different algorithms. Herein, we examined the robustness of our model to another microRNA-target list predicted by TargetScan , one of the leading target prediction tools. We constructed another functionally related microRNA network by retrieving the 15,000 most significant microRNA-microRNA relationships (edges) between 541 microRNAs (nodes). Based on this microRNA network, we created another phenome-microRNAome network and obtained a comparable performance by testing the model on the 270 known microRNA-disease associations, indicating that our model isn’t limited to a specific target prediction algorithm.
Prioritizing the entire microRNAome for 1,599 disease phenotypes
Many disease microRNAs have been identified over the past decade. However, the majority of diseases in the OMIM database aren’t associated with any microRNA. One reason is that no sufficient efforts have been made to decipher potential roles of microRNAs in those diseases. To provide testable hypotheses to guide future experiments, it is important to computationally infer possible microRNA-disease associations for diseases of interest.
Two disease phenotypes were defined to be similar if they have a phenotypic similarity score no less than 0.3 . We thus obtained 1,599 disease phenotypes, which are similar to at least one of the disease phenotypes in the benchmark dataset (see Additional file 1). We prioritized the entire microRNAome for 1,599 disease phenotypes according to score derived from the scoring system. In addition, the top 100 microRNAs for each of the 1,599 phenotypes are publicly released to facilitate the discovery of disease microRNAs (see Additional file 2).
Case study: breast cancer
We presented a case study for breast cancer, which is one of the most commonly occurring cancers among women and accounts for 22% of all female cancers. We prioritized all microRNAs for breast cancer. Among the top 100 microRNAs, 17 have been confirmed to contribute to the development of breast cancer, and 13 were verified to be deregulated in breast cancer cells. By literature retrieval, we provided more supporting evidence in Additional file 3. For example, Reddy et al. found that miR-7 inhibits p21-activated kinase 1 (Pak1) expression, a widely up-regulated signaling kinase in multiple human cancers, and the miR-7 introduction inhibits the motility, invasiveness, anchorage-independent growth and tumorigenic potential of highly invasive breast cancer cells . Foekens et al. also linked miR-7 to breast cancer aggressiveness . In addition, Scott et al. found that miR-125b is down-regulated in breast cancer and miR-125a or miR-125b-overexpressing SKBR3 cells displayed diminished plating and anchorage-dependent growth in addition to markedly reduced cell migration and invasion capacities.
We demonstrated that the method we proposed achieved good performance in recovering known, experimentally verified microRNA-disease associations. Using the model, we prioritized the entire microRNAome for 1,599 diseases, most of which have not been linked to any microRNAs. The power of our model can be attributed to several factors. First, we constructed a functionally related microRNA network, which can capture the biological characteristics of some microRNAs that tend to exert the same or similar functions by the inhibition of common target genes in a coordinated manner. Second, we took full advantage of large-scale phenotype similarity score information, whose significance has been confirmed in several previous studies aiming at the identification of disease-related protein-coding genes [1, 12]. Third, we used experimentally verified microRNA-disease associations, which allow connecting the human disease network with the microRNA network, and therefore provide underlying knowledge for the role of microRNA in disease pathogenesis.
There are several potential limitations. First, the known experimentally verified microRNA-disease associations were insufficient. Second, the functionally related microRNA network was constructed based on the standpoint that two microRNAs are functionally related if the number of shared target genes is statistically significant. In reality, two microRNAs may be functionally related when their target genes reside in the same cellular pathways or functional modules [43, 57], rather than overlap significantly. Therefore, integrating other bioinformatics sources such as Gene Ontology annotation and protein-protein interaction network data might improve model performance. In addition, modeling rules connecting phenotype with microRNA network may represent an important step on the path of the emerging field of “network medicine” [21, 58].
Evidence continually reinforces the notion that functionally related protein-coding genes are usually associated with phenotypically similar diseases. Based on this notion, many innovative methodologies have been proposed to predict or prioritize protein-coding genes for complex diseases [1–3, 7, 11, 12]. In this study, we studied the functional correlation of microRNAs and found that disease pairs associated with common microRNAs were phenotypically more similar, and the microRNA pairs linked to common diseases were functionally more related. We further constructed an integrated phenome-microRNAome network, through which we devised a method that can recover the known experimentally verified microRNA-disease associations and prioritize the entire microRNAome for 1,599 diseases. The top 100 microRNAs for each of the 1,599 diseases are released publicly, which will provide testable hypotheses to guide further experiments and contribute to the identification of true disease-related microRNAs.
We downloaded the disease phenotype similarity scores from the MimMiner , developed by Driel et al. who computed a phenotype similarity score for each phenotype pair by the text mining analysis of their phenotype descriptions in the Online Mendelian Inheritance in Man (OMIM) database . The disease phenotype network was constructed based on the similarity score. Two phenotypes were considered to be similar and were linked by an edge if their similarity score was no less than 0.3. The similarity score is equal to 1 if two phenotypes are identical. The phenotypic similarity score has been successfully used to predict or prioritize disease-related protein-coding genes [3, 12].
PITA  is a leading microRNA target prediction approach that considers multiple factors, such as seed pairing, site number, overall predicted pairing stability and predicted site accessibility. We downloaded the PITA target catalog version 6 (3/15 flank ALL 31-Aug-08) and retrieved 145,872 predicted associations between 670 microRNAs and 14,826 target genes with a score less than -10.0, a threshold suggested by PITA, In addition, we downloaded 205,587 associations between 675 microRNAs and 11,758 target genes predicted by TargetScan (version5.1, conserved sites) , another leading target prediction algorithm.
miR2Disease  and HMDD  databases provide comprehensive resources for microRNA deregulation in human disease. From these databases, we selected 270 high-quality experimentally verified microRNA-disease associations that microRNA deregulation has been experimentally verified to contribute to the disease development. For instance, Ma et al. reported that highly expressed miR-10b initiates tumor invasion and metastasis in breast cancer through translational inhibition of HOXD10, and eventually increases expression of RHOC, a pro-metastatic gene . The 270 associations (see Additional file 1) were used as the benchmark dataset for the performance evaluation of our model.
microRNA family dataset was retrieved from miRBase database.
The hypergeometric distribution is a discrete probability distribution that describes the number of successes in a sequence of n draws from a finite population without replacement. For example, there is a shipment of N objects in which M are defective. The hypergeometric distribution describes the probability that exactly m objects are defective in a sample of n distinct objects drawn from the shipment.
The biological interpretation of a high-scoring microRNA is that it is likely to be involved in the disease d. Here, N is the total number of microRNAs in the whole functionally related microRNA network. M is the number of microRNAs in the whole microRNA network associated with diseases that are similar to the disease d. n denotes the number of microRNAs in the corresponding microRNA module. A module is defined as a given microRNA (the center of the module) plus its direct network neighbors. m is the number of microRNAs that are associated with similar diseases and are found in the corresponding module.
The authors also thank Dr. George Malacinski and Tongbin Li for their valuable suggestions. This work is supported by the China 863 High-Tech Program 2007AA02Z302 (Y.L.), China Natural Science Foundation (60973078 to Y.W. 60671013 to Y.W., and 60901075 to G.W.), the U.S. National Institutes of Health grants AA017941 (Y.L.), and CA113001 (Y.L.). The authors also thank the wonderful gift from the 100 Voices of Hope Project.
This article has been published as part of BMC Systems Biology Volume 4 Supplement 1, 2010: Proceedings of the ISIBM International Joint Conferences on Bioinformatics, Systems Biology and Intelligent Computing (IJCBS). The full contents of the supplement are available online at http://www.biomedcentral.com/1752-0509/4?issue=S1.
- Lage K, Karlberg EO, Storling ZM, Olason PI, Pedersen AG, Rigina O, Hinsby AM, Tumer Z, Pociot F, Tommerup N, et al: A human phenome-interactome network of protein complexes implicated in genetic disorders. Nat Biotechnol. 2007, 25: 309-316. 10.1038/nbt1295View ArticlePubMedGoogle Scholar
- Kohler S, Bauer S, Horn D, Robinson PN: Walking the interactome for prioritization of candidate disease genes. Am J Hum Genet. 2008, 82: 949-958. 10.1016/j.ajhg.2008.02.013PubMed CentralView ArticlePubMedGoogle Scholar
- Ala U, Piro RM, Grassi E, Damasco C, Silengo L, Oti M, Provero P, Di Cunto F: Prediction of human disease genes by human-mouse conserved coexpression analysis. PLoS Comput Biol. 2008, 4: e1000043- 10.1371/journal.pcbi.1000043PubMed CentralView ArticlePubMedGoogle Scholar
- Gaulton KJ, Mohlke KL, Vision TJ: A computational system to select candidate genes for complex human traits. Bioinformatics. 2007, 23: 1132-1140. 10.1093/bioinformatics/btm001View ArticlePubMedGoogle Scholar
- George RA, Liu JY, Feng LL, Bryson-Richardson RJ, Fatkin D, Wouters MA: Analysis of protein sequence and interaction data for candidate disease gene prediction. Nucleic Acids Res. 2006, 34: e130- 10.1093/nar/gkl707PubMed CentralView ArticlePubMedGoogle Scholar
- Goh KI, Cusick ME, Valle D, Childs B, Vidal M, Barabasi AL: The human disease network. Proc Natl Acad Sci U S A. 2007, 104: 8685-8690. 10.1073/pnas.0701361104PubMed CentralView ArticlePubMedGoogle Scholar
- Franke L, van Bakel H, Fokkens L, de Jong ED, Egmont-Petersen M, Wijmenga C: Reconstruction of a functional human gene network, with an application for prioritizing positional candidate genes. Am J Hum Genet. 2006, 78: 1011-1025. 10.1086/504300PubMed CentralView ArticlePubMedGoogle Scholar
- Scharfe C, Lu HH, Neuenburg JK, Allen EA, Li GC, Klopstock T, Cowan TM, Enns GM, Davis RW: Mapping gene associations in human mitochondria using clinical disease phenotypes. PLoS Comput Biol. 2009, 5: e1000374- 10.1371/journal.pcbi.1000374PubMed CentralView ArticlePubMedGoogle Scholar
- Adie EA, Adams RR, Evans KL, Porteous DJ, Pickard BS: Speeding disease gene discovery by sequence based candidate prioritization. BMC Bioinformatics. 2005, 6: 55- 10.1186/1471-2105-6-55PubMed CentralView ArticlePubMedGoogle Scholar
- Freudenberg J, Propping P: A similarity-based method for genome-wide prediction of disease-relevant human genes. Bioinformatics. 2002, 18 (Suppl 2): S110-115.View ArticlePubMedGoogle Scholar
- Oti M, Snel B, Huynen MA, Brunner HG: Predicting disease genes using protein-protein interactions. Journal of Medical Genetics. 2006, 43:Google Scholar
- Wu X, Jiang R, Zhang MQ, Li S: Network-based global inference of human disease genes. Mol Syst Biol. 2008, 4: 189- 10.1038/msb.2008.27PubMed CentralView ArticlePubMedGoogle Scholar
- Aerts S, Lambrechts D, Maity S, Van Loo P, Coessens B, De Smet F, Tranchevent LC, De Moor B, Marynen P, Hassan B, et al: Gene prioritization through genomic data fusion (vol 24, pg 537, 2006). Nature Biotechnology. 2006, 24: 719-719. 10.1038/nbt0606-719d.View ArticleGoogle Scholar
- Lopez-Bigas N, Ouzounis CA: Genome-wide identification of genes likely to be involved in human genetic disease. Nucleic Acids Research. 2004, 32: 3108-3114. 10.1093/nar/gkh605PubMed CentralView ArticlePubMedGoogle Scholar
- Radivojac P, Peng K, Clark WT, Peters BJ, Mohan A, Boyle SM, Mooney SD: An integrated approach to inferring gene-disease associations in humans. Proteins. 2008, 72: 1030-1037. 10.1002/prot.21989PubMed CentralView ArticlePubMedGoogle Scholar
- Perez-Iratxeta C, Wjst M, Bork P, Andrade MA: G2D: a tool for mining genes associated with disease. BMC Genet. 2005, 6: 45- 10.1186/1471-2156-6-45PubMed CentralView ArticlePubMedGoogle Scholar
- Adie EA, Adams RR, Evans KL, Porteous DJ, Pickard BS: SUSPECTS: enabling fast and effective prioritization of positional candidates. Bioinformatics. 2006, 22: 773-774. 10.1093/bioinformatics/btk031View ArticlePubMedGoogle Scholar
- Perez-Iratxeta C, Bork P, Andrade MA: Association of genes to genetically inherited diseases using data mining. Nature Genetics. 2002, 31: 316-319.PubMedGoogle Scholar
- van Driel MA, Bruggeman J, Vriend G, Brunner HG, Leunissen JA: A text-mining analysis of the human phenome. Eur J Hum Genet. 2006, 14: 535-542. 10.1038/sj.ejhg.5201585View ArticlePubMedGoogle Scholar
- Oti M, Huynen MA, Brunner HG: Phenome connections. Trends Genet. 2008, 24: 103-106. 10.1016/j.tig.2007.12.005View ArticlePubMedGoogle Scholar
- Zanzoni A, Soler-Lopez M, Aloy P: A network medicine approach to human disease. FEBS Lett. 2009Google Scholar
- Oti M, Brunner HG: The modular nature of genetic diseases. Clin Genet. 2007, 71: 1-11. 10.1111/j.1399-0004.2006.00708.xView ArticlePubMedGoogle Scholar
- Linghu B, Snitkin ES, Hu Z, Xia Y, Delisi C: Genome-wide prioritization of disease genes and identification of disease-disease associations from an integrated human functional linkage network. Genome Biol. 2009, 10: R91- 10.1186/gb-2009-10-9-r91PubMed CentralView ArticlePubMedGoogle Scholar
- Calin GA, Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 2006, 6: 857-866. 10.1038/nrc1997View ArticlePubMedGoogle Scholar
- Esquela-Kerscher A, Trang P, Wiggins JF, Patrawala L, Cheng A, Ford L, Weidhaas JB, Brown D, Bader AG, Slack FJ: The let-7 microRNA reduces tumor growth in mouse models of lung cancer. Cell Cycle. 2008, 7: 759-764.View ArticlePubMedGoogle Scholar
- Huang Q, Gumireddy K, Schrier M, le Sage C, Nagel R, Nair S, Egan DA, Li A, Huang G, Klein-Szanto AJ, et al: The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis. Nat Cell Biol. 2008, 10: 202-210. 10.1038/ncb1681View ArticlePubMedGoogle Scholar
- Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, Sweet-Cordero A, Ebert BL, Mak RH, Ferrando AA, et al: MicroRNA expression profiles classify human cancers. Nature. 2005, 435: 834-838. 10.1038/nature03702View ArticlePubMedGoogle Scholar
- Markou A, Tsaroucha EG, Kaklamanis L, Fotinou M, Georgoulias V, Lianidou ES: Prognostic value of mature microRNA-21 and microRNA-205 overexpression in non-small cell lung cancer by quantitative real-time RT-PCR. Clin Chem. 2008, 54: 1696-1704. 10.1373/clinchem.2007.101741View ArticlePubMedGoogle Scholar
- Nasser MW, Datta J, Nuovo G, Kutay H, Motiwala T, Majumder S, Wang B, Suster S, Jacob ST, Ghoshal K: Down-regulation of micro-RNA-1 (miR-1) in lung cancer. Suppression of tumorigenic property of lung cancer cells and their sensitization to doxorubicin-induced apoptosis by miR-1. J Biol Chem. 2008, 283: 33394-33405. 10.1074/jbc.M804788200PubMed CentralView ArticlePubMedGoogle Scholar
- Slack FJ, Weidhaas JB: MicroRNA in cancer prognosis. N Engl J Med. 2008, 359: 2720-2722. 10.1056/NEJMe0808667View ArticlePubMedGoogle Scholar
- Yang B, Lin H, Xiao J, Lu Y, Luo X, Li B, Zhang Y, Xu C, Bai Y, Wang H, et al: The muscle-specific microRNA miR-1 regulates cardiac arrhythmogenic potential by targeting GJA1 and KCNJ2. Nat Med. 2007, 13: 486-491. 10.1038/nm1569View ArticlePubMedGoogle Scholar
- Yu SL, Chen HY, Chang GC, Chen CY, Chen HW, Singh S, Cheng CL, Yu CJ, Lee YC, Chen HS, et al: MicroRNA signature predicts survival and relapse in lung cancer. Cancer Cell. 2008, 13: 48-57. 10.1016/j.ccr.2007.12.008View ArticlePubMedGoogle Scholar
- Chen RW, Bemis LT, Amato CM, Myint H, Tran H, Birks DK, Eckhardt SG, Robinson WA: Truncation in CCND1 mRNA alters miR-16-1 regulation in mantle cell lymphoma. Blood. 2008, 112: 822-829. 10.1182/blood-2008-03-142182PubMed CentralView ArticlePubMedGoogle Scholar
- Duisters RF, Tijsen AJ, Schroen B, Leenders JJ, Lentink V, van der Made I, Herias V, van Leeuwen RE, Schellings MW, Barenbrug P, et al: miR-133 and miR-30 regulate connective tissue growth factor: implications for a role of microRNAs in myocardial matrix remodeling. Circ Res. 2009, 104: 170-178, 176p following 178. 10.1161/CIRCRESAHA.108.182535View ArticlePubMedGoogle Scholar
- Miller TE, Ghoshal K, Ramaswamy B, Roy S, Datta J, Shapiro CL, Jacob S, Majumder S: MicroRNA-221/222 confers tamoxifen resistance in breast cancer by targeting p27Kip1. J Biol Chem. 2008, 283: 29897-29903. 10.1074/jbc.M804612200PubMed CentralView ArticlePubMedGoogle Scholar
- Weinberg MS, Wood MJ: Short non-coding RNA biology and neurodegenerative disorders: novel disease targets and therapeutics. Hum Mol Genet. 2009, 18: R27-39. 10.1093/hmg/ddp070PubMed CentralView ArticlePubMedGoogle Scholar
- Xin FX, Li M, Balch C, Thomson M, Fan MY, Liu Y, Hammond SM, Kim S, Nephew KP: Computational analysis of microRNA profiles and their target genes suggests significant involvement in breast cancer antiestrogen resistance. Bioinformatics. 2009, 25: 430-434. 10.1093/bioinformatics/btn646PubMed CentralView ArticlePubMedGoogle Scholar
- Jiang Q, Wang Y, Hao Y, Juan L, Teng M, Zhang X, Li M, Wang G, Liu Y: miR2Disease: a manually curated database for microRNA deregulation in human disease. Nucleic Acids Res. 2009, 37: D98-104. 10.1093/nar/gkn714PubMed CentralView ArticlePubMedGoogle Scholar
- Lu M, Zhang Q, Deng M, Miao J, Guo Y, Gao W, Cui Q: An analysis of human microRNA and disease associations. PLoS ONE. 2008, 3: e3420- 10.1371/journal.pone.0003420PubMed CentralView ArticlePubMedGoogle Scholar
- Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004, 116: 281-297. 10.1016/S0092-8674(04)00045-5View ArticlePubMedGoogle Scholar
- Georges SA, Biery MC, Kim SY, Schelter JM, Guo J, Chang AN, Jackson AL, Carleton MO, Linsley PS, Cleary MA, Chau BN: Coordinated regulation of cell cycle transcripts by p53-Inducible microRNAs, miR-192 and miR-215. Cancer Res. 2008, 68: 10105-10112. 10.1158/0008-5472.CAN-08-1846View ArticlePubMedGoogle Scholar
- Lee Y, Samaco RC, Gatchel JR, Thaller C, Orr HT, Zoghbi HY: miR-19, miR-101 and miR-130 co-regulate ATXN1 levels to potentially modulate SCA1 pathogenesis. Nat Neurosci. 2008, 11: 1137-1139. 10.1038/nn.2183PubMed CentralView ArticlePubMedGoogle Scholar
- Packer AN, Xing Y, Harper SQ, Jones L, Davidson BL: The bifunctional microRNA miR-9/miR-9* regulates REST and CoREST and is downregulated in Huntington's disease. J Neurosci. 2008, 28: 14341-14346. 10.1523/JNEUROSCI.2390-08.2008PubMed CentralView ArticlePubMedGoogle Scholar
- Zhao JJ, Lin JH, Yang H, Kong W, He LL, Ma X, Coppola D, Cheng JQ: MicroRNA-221/222 Negatively Regulates Estrogen Receptor alpha and Is Associated with Tamoxifen Resistance in Breast Cancer. Journal of Biological Chemistry. 2008, 283: 31079-31086. 10.1074/jbc.M806041200PubMed CentralView ArticlePubMedGoogle Scholar
- Mayoral RJ, Pipkin ME, Pachkov M, van Nimwegen E, Rao A, Monticelli S: MicroRNA-221-222 regulate the cell cycle in mast cells. J Immunol. 2009, 182: 433-445.PubMed CentralView ArticlePubMedGoogle Scholar
- Gabor B, Katalin O, Farkas IJ: Human microRNAs co-silence in well-separated groups and have different predicted essentialities. Bioinformatics. 2009, 25: 1063-1069. 10.1093/bioinformatics/btp018View ArticleGoogle Scholar
- Xu JZ, Wong CW: A computational screen for mouse signaling pathways targeted by microRNA clusters. Rna-a Publication of the Rna Society. 2008, 14: 1276-1283.View ArticleGoogle Scholar
- Mookherjee S, Sinha M, Mukhopadhyay S, Bhattacharyya N, Mohanty P: MicroRNA Interaction network in human: implications of clustered microRNA in biological pathways and genetic diseases. Arxiv preprint arXiv:09014211. 2009Google Scholar
- Ideker T, Sharan R: Protein networks in disease. Genome Res. 2008, 18: 644-652. 10.1101/gr.071852.107PubMed CentralView ArticlePubMedGoogle Scholar
- Barabasi AL, Oltvai ZN: Network biology: understanding the cell's functional organization. Nat Rev Genet. 2004, 5: 101-113. 10.1038/nrg1272View ArticlePubMedGoogle Scholar
- Benjamini Y, Hochberg Y: Controlling the False Discovery Rate - a Practical and Powerful Approach to Multiple Testing. Journal of the Royal Statistical Society Series B-Methodological. 1995, 57: 289-300.Google Scholar
- Benjamini Y, Yekutieli D: The control of the false discovery rate in multiple testing under dependency. Annals of Statistics. 2001, 29: 1165-1188. 10.1214/aos/1013699998.View ArticleGoogle Scholar
- Kertesz M, Iovino N, Unnerstall U, Gaul U, Segal E: The role of site accessibility in microRNA target recognition. Nat Genet. 2007, 39: 1278-1284. 10.1038/ng2135View ArticlePubMedGoogle Scholar
- Lewis BP, Shih IH, Jones-Rhoades MW, Bartel DP, Burge CB: Prediction of mammalian microRNA targets. Cell. 2003, 115: 787-798. 10.1016/S0092-8674(03)01018-3View ArticlePubMedGoogle Scholar
- Reddy SD, Ohshiro K, Rayala SK, Kumar R: MicroRNA-7, a homeobox D10 target, inhibits p21-activated kinase 1 and regulates its functions. Cancer Res. 2008, 68: 8195-8200. 10.1158/0008-5472.CAN-08-2103PubMed CentralView ArticlePubMedGoogle Scholar
- Foekens JA, Sieuwerts AM, Smid M, Look MP, de Weerd V, Boersma AW, Klijn JG, Wiemer EA, Martens JW: Four miRNAs associated with aggressiveness of lymph node-negative, estrogen receptor-positive human breast cancer. Proc Natl Acad Sci U S A. 2008, 105: 13021-13026. 10.1073/pnas.0803304105PubMed CentralView ArticlePubMedGoogle Scholar
- Crosby ME, Kulshreshtha R, Ivan M, Glazer PM: MicroRNA regulation of DNA repair gene expression in hypoxic stress. Cancer Res. 2009, 69: 1221-1229. 10.1158/0008-5472.CAN-08-2516PubMed CentralView ArticlePubMedGoogle Scholar
- Barabasi AL: Network medicine--from obesity to the "diseasome". N Engl J Med. 2007, 357: 404-407. 10.1056/NEJMe078114View ArticlePubMedGoogle Scholar
- Hamosh A, Scott AF, Amberger JS, Bocchini CA, McKusick VA: Online Mendelian Inheritance in Man (OMIM), a knowledgebase of human genes and genetic disorders. Nucleic Acids Res. 2005, 33: D514-517. 10.1093/nar/gki033PubMed CentralView ArticlePubMedGoogle Scholar
- Ma L, Teruya-Feldstein J, Weinberg RA: Tumour invasion and metastasis initiated by microRNA 10b in breast cancer. Nature. 2007, 449: 682-U682. 10.1038/nature06174View ArticlePubMedGoogle Scholar
- Griffiths-Jones S, Saini HK, van Dongen S, Enright AJ: miRBase: tools for microRNA genomics. Nucleic Acids Res. 2008, 36: D154-158. 10.1093/nar/gkm952PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.