The plasticity of TGF-βsignaling
BMC Systems Biology volume 5, Article number: 184 (2011)
The family of TGF-β ligands is large and its members are involved in many different signaling processes. These signaling processes strongly differ in type with TGF-β ligands eliciting both sustained or transient responses. Members of the TGF-β family can also act as morphogen and cellular responses would then be expected to provide a direct read-out of the extracellular ligand concentration. A number of different models have been proposed to reconcile these different behaviours. We were interested to define the set of minimal modifications that are required to change the type of signal processing in the TGF-β signaling network.
To define the key aspects for signaling plasticity we focused on the core of the TGF-β signaling network. With the help of a parameter screen we identified ranges of kinetic parameters and protein concentrations that give rise to transient, sustained, or oscillatory responses to constant stimuli, as well as those parameter ranges that enable a proportional response to time-varying ligand concentrations (as expected in the read-out of morphogens). A combination of a strong negative feedback and fast shuttling to the nucleus biases signaling to a transient rather than a sustained response, while oscillations were obtained if ligand binding to the receptor is weak and the turn-over of the I-Smad is fast. A proportional read-out required inefficient receptor activation in addition to a low affinity of receptor-ligand binding. We find that targeted modification of single parameters suffices to alter the response type. The intensity of a constant signal (i.e. the ligand concentration), on the other hand, affected only the strength but not the type of the response.
The architecture of the TGF-β pathway enables the observed signaling plasticity. The observed range of signaling outputs to TGF-β ligand in different cell types and under different conditions can be explained with differences in cellular protein concentrations and with changes in effective rate constants due to cross-talk with other signaling pathways. It will be interesting to uncover the exact cellular differences as well as the details of the cross-talks in future work.
Transforming growth factor beta (TGF-β) signaling has been implicated as an important regulator of almost all major cell behaviors, including proliferation, differentiation, cell death, and motility . Which response is induced or repressed depends on the cell type and context in which the signal is received.
The complexity of the biological outcomes elicited by TGF-β stands in stark contrast to the apparent simplicity of the signaling cascade. In response to TGF-β, type 1 (ALKs 1-7 in humans) and type 2 receptors (ActR-IIA, ActR-IIB, BMPR-II, AMHR-II and TbR-II, in humans) form complexes and the constitutively active type 2 serine/threonine kinase phosphorylates the type 1 receptor. The activated type 1 receptor transduces the signal into the cell by phosphorylating the regulatory Smads (R-Smad: Smad 2 and 3 in case of the TGF-β subfamily, and Smad 1, 5 and 8 for the BMP subfamily). Once activated R-Smads form homomeric complexes and heteromeric complexes with the common Smad, Co-Smad (Smad 4) . Smads continuously shuttle between nucleus and cytoplasm . TGF-β signaling biases Smad localisation to the nucleus  where Smad complexes associate with chromatin and regulate the transcription of hundreds of genes . Signal termination is achieved through continuous dephosphorylation of the R-Smad (mainly in the nucleus ) and induction of inhibitory Smads (I-Smad: Smad 6 for the BMP subfamily, and Smad7 for the TGF-β subfamily). I-Smads act through diverse mechanisms: by targeting active receptor for proteasomal degradation [6, 7], inducing receptor dephosphorylation  and competing with R-Smad for the receptor binding site . Rapid shuttling and inactivation enables a continuous sensing of the extracellular ligand concentrations . This is likely to be particular important when members of the TGF-β ligand family acts as morphogen and determine cell-fate in a concentration-dependent manner.
Beyond the core components of this signaling pathway many other factors modulate the signal and thereby contribute to the versality of the response. At the membrane level, the access to receptor is controlled by soluble proteins that sequester TGF-β ligand (i.e. decorin) , and by membrane-bound co-receptors that promote binding (i.e. betaglycan) . The receptor activity is further regulated by several receptor internalization routes , and by receptor turnover. Intracellularly, many processes require auxiliary proteins (i.e. SARA for the binding of R-Smad to the receptor and Schnurri for the binding of the R-Smad/Co-Smad complex to the DNA binding element) [2, 13]. The restriction of those auxiliary factors to specific cell-types will make the response cell context dependent . Diversity can also be generated by the huge number of different possible combinations of type 1 and type 2 receptors  and the multiple crosstalks of the TGF-β signaling cascade with other pathways. One example of regulation by cross-talk is the phosphorylation of R-Smads in the linker region by Ras-activated MAPK , calcium-calmodulin-dependent protein kinase II  or CDKs . Phosphorylation reduces the transcriptional activity of the R-Smad .
Several mathematical models have been developed to gain further insights into the complex TGF-β-dependent signaling network . An early model by Clarke and co-workers (2006)  focused on the nuclear accumulation of Smad complexes. Their conclusion on the central role of the imbalance between R-Smad phosphorylation and dephosphorylation rates were confirmed by a more detailed model by Schmierer et al. (2008) . Experiments suggest that the duration of the response to a ligand stimulation strongly impacts on the cellular response. Thus epithelial cells that elicit sustained nuclear Smad complex accumulation respond to TGF-β with cell growth arrest, whereas pancreatic tumor cells that elicit a transient response continue proliferating (while keeping other TGF-β induced behaviors) . Much theoretical work therefore focused on how sustained, transient, or switch-like responses could be obtained by adjusting the receptor dynamics, ligand depletion, and the I-Smad dependent negative feedback. Melke et al. (2006)  focused on the potential role of I-Smads in generating transient responses while Vilar et al. (2006) focused on the receptor dynamics to explain the occurrence of both transient and sustained responses. Zi et al. (2007)  included a simple model of the Smad dynamics and highlighted the importance of the balance between clathrin-dependent endocytosis and non-clathrin mediated endocytosis. All pathway elements were finally brought together by Chung et al. (2009)  in a more comprehensive model, used to examine the contradictory roles of TGF-β in cancer progression. Lately Zi et al. (2011)  published a study that highlights the potential of TGF-β ligand depletion in converting short-term graded signaling responses into long-term switch-like responses. Unlike for other pathways oscillations have not yet reported for the TGF-β signaling pathway [26, 27]. TGF-β type ligands are also acting as morphogens, and the response to these appears to be proportional. Recently, Paulsen and co-workers published a study on the impact of synexpression of the feedback inhibitors BAMBI, Smad6, and Smad7 on the read-out of morphogen gradients during embryogenesis .
While the many published studies explain the different behaviours for the different situations for which they are observed and highlight the many mechanisms that enable the different response types it remains largely unclear how easily the response type can be changed. We wondered how the TGF-β signaling pathway accomplishes the flexibility in its responses and which and how many parameters have to be altered for cells to respond differently. To efficiently explore the canonical response we focused on the core signaling architecture, and did not consider the detailed receptor dynamics and cross-talks in the model; they are included indirectly through the parameters that they modulate. We explored the response types and in particular changes in the response type as we explored the parameter values within biologically meaningful ranges. We find that relatively small changes in single parameters can alter the response. Cellular protein concentrations are a particular powerful point of control and this explains how different cell types can show different responses. Importantly we also identify key parameters that affect the response and we can relate these to observed points of cross-talk between signaling pathways. The particular architecture of the TGF-β network thus allows for the great flexibility in the response.
Several models for the TGF-β signaling network have been developed that focus on different aspects of the TGF-β signaling network, i.e. the receptor dynamics [23, 29], the shuttling between the cytoplasm and the nucleus , and the negative feedback via the I-Smad (Smad7/Dad) . These different aspects have lately been combined in a model that addresses differences in TGF-β signaling between normal and cancerous cells . The models of the TGF-β signaling pathway showed that stimulation could result in either transient and sustained responses dependent on the choice of parameters [3, 22–25, 29]. Transient responses could be obtained through complex receptor dynamic , the I-Smad-mediated negative feedback [3, 22], or ligand depletion . Negative feedbacks can in principle also give rise to oscillatory behaviour. We wondered whether all three qualitative behaviours (sustained, transient, or oscillatory response) could be obtained already with the most simple intracellular feedback mechanism, and how these behaviours would depend on the parameters. Since the more complex interactions (that we ignore) effectively modulate the parameter values in our model an in-depth understanding of the parameter dependencies in the simple model should also enable a better understanding of the complex network interactions that are found in the cell. The different response types can also (trivially) be obtained by modulating the protein concentrations accordingly. We, however, keep the concentrations of receptors, ligand, R-Smad and Co-Smad constant and thus include these effects only indirectly as changes in the effective binding rates.
Accordingly, we formulated a detailed model of TGF-β signaling that focused on the negative feedback, but did not include any complex receptor dynamics as these require changes in the receptor and ligand concentrations. Our model describes the dynamics of TGF-β ligand (TGF-β), receptor (TGFβR), regulatory R-Smads (denoted simply Smad), Co-Smads, I-Smads, their complexes as well as the expression intermediates of the I-Smad. Importantly, we include two compartments, the nucleus and the cytoplasm, and the Smad and Co-Smad complexes can shuttle between the two compartments as first described in . The regulatory interactions are summarized in Figure 1 (a SBML file is provided in Additional file 1). Thus the ligand TGF-β reversibly binds to the TGF-β receptor (reactions 1 and 2 in Figure 1), which is then phosphorylated to become fully active (3 and 4). The active receptor induces phosphorylation of R-Smad (7), which in turn can reversibly dimerize or form a complex with Co-Smad (10 and 11). Those two reactions can take place either in the cytoplasm or in the nucleus and the five species Smad, phosphorylated Smad, Co-Smad, homodimers and heterodimers can shuttle from the cytoplasm to the nucleus and back (8, 9 and 12). Nuclear Smad/Co-Smadf complexes act as transcription factors and trigger the transcription of I-Smad mRNA in the nucleus (14 and 15). The I-Smad mRNA then shuttles to the cytoplasm (16), where it can be degraded (17) or translated into I-Smad (18). I-Smad mediates a negative feedback by sequestering the active receptor (5 and 6) and can be degraded (19). The response to a stimulus by TGF-β ligand is a change in the transcriptional activity, monitored as the nuclear concentration of Smad/Co-Smad complexes.
We translated those interactions into sets of ODEs using the law of mass action where appropriate. To reduce the complexity of the model we also employed Hill functions to describe the regulation by cooperative interactions. To efficiently investigate the impact of changes in total concentration of receptors, R-Smad, and Co-Smad we used a total concentration rather than production and degradation rates for these species.
To respond to TGF-β cells must be able to detect changes in the ligand concentration and convert the differences into different transcriptional responses. Transcriptional activity is determined by the concentration of transcription factors in the nucleus. We therefore monitor the nuclear concentration of R-Smad/Co-Smad complexes as a measure of transcriptional activity, in response to a change in the extracellular TGF-β concentration.
Parameter screening and simulations
We are interested in the signaling capacity of the TGF-β pathway within its physiological limits. These physiological limits are set by the plausible range that the parameter values can take. We established a likely range for each parameter value based on available data and estimates (Additional file 2, Table S1 and Table S2). While previous measurements and estimates are necessarily of limited accuracy and differences are likely to exist between different cells and different cell types [3, 22–24, 29] we expect that basing ourselves on the available data will not too much distort the ranges that we screen. Most parameters were varied over 3 or 4 orders of magnitude, centered around the mean of values found in the literature. Since there are no good estimates for the I-Smad expression rates k14 and k15 were varied over 5 orders of magnitude. The rates of phosphorylation and dephosphorylation (k7 and k13) were varied only over two orders of magnitude because a large fraction of the simulations failed when these rate constants were varied over a wider range. To avoid a bias to the few parameter sets that do not lead to extreme dynamics we had to constrain these two parameters to only vary over two orders of magnitude. To determine the possible range of pathway responses to a defined stimulus, we carried out 106 independent simulations with parameter values randomly picked from a uniform logarithmic distribution of parameter values within the set ranges (as discussed in Geier et al. ) and compared the predicted nuclear concentration of R-Smad/Co-Smad complexes in response to the ligand stimulus. In a first step, we let the system equilibrate for 1 hour with almost no ligand (concentration of 10-6 pM to avoid failure of the solver) and initial cellular concentrations TGFβR = 1 nM, Smad = 60 nM and Co-Smad = 100 nM. We then used the steady-state value of the first step and solved the simulations for 10 hours with a constant ligand concentration of 200 pM. Using MATLAB's ode15 s routine the 106 simulations took in total approximately 140 hours of CPU time.
Criteria to define the different TGF-βsignaling responses
In response to ligand exposure we observed five different qualitative responses, i.e. unresponsive, sustained, transient, dampened oscillatory or sustained oscillatory responses (Figure 2). Additional file 3, Fig. S1, S2, S3, and S4 show the evolution of the concentration of each species over time in a representative transient and a representative sustained response (Additional file 2, Table S3). To define the parameter dependency of the different response types we made the following definitions: We speak of unresponsiveness if the concentration of nuclear R-Smad/Co-Smad complexes remains below a chosen threshold θ within ten hours of stimulation. Accordingly we speak of responsiveness if the concentration exceeds the threshold concentration θ, and here we distinguished four distinct behaviours, inspired by the work of Ma et al. and based on the subsequent dynamics:
1. Sustained response
After the initial peak the response must retain at least 90% of its maximal value (called Opeak). To exclude slowly increasing responses we further require that 90% of the peak value Opeak is reached within less than 7200 s (2 hours).
2. Transient response
After the initial peak the response must drop to levels lower than 10% of its peak value Opeak within less than two hours and the final value (after 10 hours) (called Oend) must be lower than 0.1 × θ.
After the initial peak the amplitude (difference between the local maximum and local minimum) must exceed 0.1 × θ at least 4 times.
3.1 Dampened oscillations
The fifth amplitude must be less than half the second amplitude.
3.2 Sustained oscillations
The fifth amplitude must be higher than half the second amplitude.
We characterized the long-term behaviour of oscillations based on the relative amplitudes of the second and fifth peak because the first peak can be particularly high (Figure 2D), and most dampened simulations have no more than five peaks.
Quantitative data on the physiological concentrations of the cellular proteins and the transcription factor complex (nuclear Smad/CoSmad complex) do not exist, and we therefore had to set our detection threshold arbitrarily to θ = 10 pM when analysing a unique constant stimulus with 200 pM TGF-β ligand. When the response to several ligand concentrations or with several protein concentrations was studied we used the maximal response value as θ. Simulations were run for 10 hours. In case if oscillations, if the amplitude of oscillations was still larger than 0.1 × θ after 10 hours, the simulation was continued until the oscillations vanished, but for a maximum 100 hours. In this way we avoid any impact of period length on the classification of oscillations, and the length of the period indeed does not bias our characterisation of oscillations to dampened or sustained oscillatory behaviour (Additional file 3, Fig. S5C). The time thresholds 2 hours and 10 hours were chosen based on experimental data .
Results and Discussion
In response to a sustained stimulus (200 pM ligand) our simple model for TGF-β signaling can give rise to sustained (Figure 2A), transient (Figure 2B), or oscillatory (Figure 2C, D) responses. The sustained/transient distinction is particularly relevant, as it has been shown that those two qualitative behaviors are related to the growth inhibitory effect of TGF-β . To better understand the conditions for these different behaviours we sought to identify parameter families that would give rise to a certain response type. We hoped that a comparison of those families would reveal the critical parameters that determine the response type. To that end we screened a large number of parameter sets and classified them according to their responses as described in detail in the Materials and Methods section.
Parameter-dependent distinct qualitative responses
Our criteria in Figure 2 are very strict (i.e. the speed of responses is an important criterium) and there is a wide undefined range between sustained and transient responses. As a consequence most parameter sets (57.5%) do not fall into any of the defined categories (Additional file 3, Fig. S5A, black sets). Of those that can be classified most (25.5% of the parameter sets tested) led to no response (Additional file 3, Fig. S5A, marked in grey). Among the "responsive" parameter sets most lead to sustained responses (14.8% of the parameter sets tested) (Additional file 3, Fig. S5A, marked in red) while transient responses are observed less frequently (2.2%of the parameter sets tested) (Additional file 3, Fig. S5A, marked in green). All three behaviors have previously been observed in various models of TGF-β signaling. We find that in addition in a minority of cases (306 simulations, i.e 0.046%) also oscillatory responses can be produced (Additional file 3, Fig. S5B). Even though the number of sets that give rise to oscillations in the concentration of nuclear transcription factor complexes is small, these may occupy a sufficiently dense subspace in the parameter space to be physiologically relevant. The oscillations can either be sustained or dampened, depending on how fast their amplitude decays (Figure 2C, D). As expected sustained oscillations have a larger number of peaks (Additional file 3, Fig. S5B). While the period of the oscillations is not biased to dampened or sustained oscillatory behaviour (Additional file 3, Fig. S5C), the duration (the time until oscillations vanish) depends on both the number of peaks and the duration that tends to be higher for sustained oscillations (Additional file 3, Fig. S5D).
Oscillatory behavior has been reported for a number of other signaling pathways (i.e. the ERK cascade ), but so far no experimental evidence exists for oscillations in the TGF-β pathway. However, standard biochemical experiments average over a large number of non-synchronized cells. If the nuclear concentration of transcription factor indeed oscillated, only sophisticated single-cell assays would reveal these.
The impact of kinetic parameters on the response type
We wondered which kinetic parameters would be critical for the different response types. Our sampling space is huge (23 parameters, with most of them sampled over 4 orders of magnitude, Additional file 2, Table S1) and we looked for parameters that would be constrained in the different response types. In Figure 3 we plot the sampled ranges in grey, and the parameter ranges that correspond to the different response types in colours. Since we are sampling from a uniform logarithmic distribution parameters that are not affecting the response type should remain uniformly logarithmically distributed in the parameter subsets. In Figure 3A we compare the parameter ranges of sustained (red) and transient (green) responses. We notice that whereas some parameter values remain (almost) uniformly distributed, others are constrained. Constrained parameters include the rates that describe the I-Smad dependent negative feedback loop (parameters k5, k6, k14, k15, k17, k18, and k19), the shuttling rate between cytoplasm and nucleus (k8), the dynamics of the Smad homo- and heterodimer formation/dissolution(k10, and k11), and the dephosphorylation of Smad (k13). Figure 4A shows the clear segregation of the "sustained" (red) and "transient" (transient) parameter sets in a plane spanned by the parameters that determine the strength of the negative feedback ((k14 × k18)/(k15 × k17 × k19) × k5/k6) and the speed of Smad dephosphorylation, k8 × (k11 × k13)/k10. To favour transient responses over the sustained responses the I-Smad dependent negative feedback must be strong and dephosphorylation of Smad must be fast. The need for rapid dephosphorylation likely arises also because of our requirement that adaptation must happen within 2 hours. We notice that the size of the parameter set that permits transient responses is considerably smaller than the parameter set that permits sustained responses. However, transient responses can also result from degradation of core signaling components and ligand which is not considered here.
Transient and oscillatory responses are similar in that the response must decay quickly in spite of the continuous presence of ligand. A similar comparison of the parameter ranges that permit transient (green) or oscillatory (blue and magenta) responses (Figure 3B) indeed reveals that similar restrictions apply (i.e. large shuttling rate between cytoplasm and nucleus, k8, and strong negative feedback, k14, k15, k17, k18, and k19). However, in case of oscillations the response restarts and in addition we indeed notice a strong restriction of the rate of ligand-receptor binding k2 in case of oscillatory responses. Figure 4B shows the clear segregation of the parameter sets that give rise to "transient" (green), dampened (blue) or sustained (purple) oscillatory responses in a plane spanned by the receptor-ligand binding rate k2 and the speed of I-Smad turn-over (k16 × k17 × k19). Oscillations are observed only when k2 is small such that ligand binds slowly to its receptor and the pool of free receptor is depleted gradually (Additional file 3, Fig. S6A). As a consequence free receptor is still available when I-Smad has downregulated the response and ligand can still trigger a further response. Conversely, if k2 is large, receptors are rapidly bound to the ligand (whose concentration is constant in this model), and once the response has terminated, there is no free receptor available to induce a new response (Additional file 3, Fig. S6B). k2, and thus the speed with which the free receptor concentration decreases, critically determine the dampening of oscillations. A 10-fold change in the value of k2 can transform sustained oscillations in highly dampened ones (compare panels A and B in Additional file 3, Fig. S6). Rapid degradation of I-Smads is important for sustained oscillations because otherwise all receptors become rapidly sequestered by I-Smad and the response is terminated. Accordingly inclusion of receptor endocytosis and recycling to the membrane (cycling) combined with the removal of the I-Smad would allow further oscillatory cycles.
Each parameter in our simple model integrates the effects of many further interactions as may also arise from cross-talk. Thus it has been shown that the phosphorylation of Smad in its linker region by Ras-activated MAPK induces a cytoplasmic retention of R-Smads , which in our system would be represented by a lower shuttling rate into the nucleus (k8). Interestingly k8 indeed strongly influenced the response type. Another parameter that appears to be important in determining the response characteristics is the binding rate of TGF-β to the receptor (k2). Our description of the processes at the cell membrane is very simple and thus k2 has also to take in account the regulation of TGF-β outside the cell by soluble sequestering factors and membrane-bound co-receptors as well as processes that affect the receptor density on the cell membrane. Those auxiliary factors play therefore a crucial role in the TGF-β pathway flexibility. We should stress that all parts of the parameter space should be readily reachable for the cell and small adjustments in the parameter values should thus be sufficient to alter the response type.
The regulatory impact of cellular protein concentrations
The kinetic rate constants of a reaction depend on the particular protein chemistry. While rate constants may be different between species, rate constants are unlikely to differ between individuals of one species and even more unlikely to differ within a single individual. However, during the development of an organism the same signaling network can elicit qualitatively different responses at different times and locations. We therefore wondered whether changes in the protein concentrations (which can be easily adjusted by an organism or result from crosstalks with other signaling pathways) would enable the required regulatory flexibility. To find parameter ranges that would permit such flexibility we repeated our previous screen with different concentrations of receptors, R-Smad or Co-Smad: for each of the three species we first carried out 3 screens where concentrations were increased or decreased from their reference concentration c0 to c0/100 or c0 × 100. We then looked for parameter sets that would permit a switch between a transient and a sustained output response as the protein concentrations changed. Our parameter sampling space is huge (23 parameters, with most of them sampled over 4 orders of magnitude, Additional file 2, Table S1) and a switch could be observed for less than 1% of the sets (Figure 5A, black bars). When we plotted the parameter ranges for which we observed switching we noted that a number of parameter ranges were restricted compared to the initial sampling range (Additional file 3, Fig. S7). We therefore wondered whether there would be particular parameter ranges for which concentration-dependent switching would be more frequent. Indeed when we reduced the sampling ranges of the parameter values (Additional file 3, Fig. S7) about 20% of the parameter sets enabled switching as the R-Smad concentration was varied, 25% as the receptor concentration was varied, and almost 30% as the Co-Smad concentration was varied (Figure 5A, grey bars). We notice that the only rates that were not restricted while enhancing the fraction of parameter sets that permit switching were the rate of ligand-TGFβR unbinding (k1) and of I-Smad mRNA export (k16). These rates thus appear to have very little influence on the overall kinetics within the screened range. We next wondered what would be the minimal change needed in protein concentration to allow the switch. To that end we carried out 9 supplementary screens for each of the three species where concentrations were increased or decreased from their reference concentration c0 over a 100-fold range in multiples of 3, i.e. c0(n) = c03 n with n = [-4, -3, ..., 3, 4]. Interestingly, while a change in the response type was observed most frequently in response to changes in the Co-Smad concentration (Figure 5A, grey bars), switches could be achieved with much smaller concentration changes when the receptor or R-Smad concentration were varied (Figure 5B). Thus only a 3-10 fold change in the receptor and R-Smad concentration was typically required while the Co-Smad concentration typically needed to be changed by 20-100-fold. The I-Smad Smad6 has indeed been reported to inhibit TGF-β signaling by sequestering the Co-Smad Smad4 in an inactive complex . It has further been argued that cross-talk between different TGF-β pathways may be integrated via a competition for Co-Smads. Based on our observations such competition would need to greatly alter the concentration of available Co-Smad to be effective and the receptor and the R-Smad would offer a more sensitive point of control. Previous models have focused on the dynamical control of the TGF-β receptor and have shown that this indeed offers great regulatory flexibility . Experiments further show that the I-Smad may also affect the turn-over rate of R-Smads and thus affect their cellular concentration .
Finally we wondered how different ligand concentrations would affect the cellular response. The impact of different TGF-β concentrations have already been studied by Clarke et al.  and Zi et al. , but there the results were strongly affected by ligand depletion since the TGF-β concentrations were allowed to go down over time because of internalization and degradation. We were interested how different, but constant, stimuli would affect the response - the effect of ligand depletion can then be deduced as response to decreasing ligand concentrations. As we varied the ligand concentration between 0.2 pM and 20 nM we noticed that only for a very small fraction of parameter sets (less than 0.5%) the response type changed qualitatively as the concentration varied. The parameter sets were not clustered and a further increase by restricting parameter ranges (as in case of the cellular protein concentrations) could not be achieved. Even though changes in the TGF-β concentration cannot switch the response type in our simulations, the duration of the response increases with increasing TGF-β concentrations as previously observed by Zi et al. . This increase was, however, insufficient to alter the response type according to our definitions.
The ligand concentration clearly affects the maximal response in our simulations, and the transcription factor activity increases with the ligand concentration until a plateau is reached (Figure 6A). In case of sustained responses (but not for transient responses) the peak value is reached more quickly at higher ligand concentrations (data not shown). The saturation curve in Figure 6A can be fitted with an exponential curve, i.e. O peak = max(O peak )(1 - exp(-x/η)) where x refers to the TGF-β ligand concentration (x = 0.2, 2, 6, 10, 15, 20, 50, 100, 150, 200, 1000, 2000 pM) and the parameter η indicates the concentration range for which the response saturates. Histograms of η (Figure 6B, C) show that the sustained response (Figure 6C) tends to saturate at lower TGF-β concentrations than transient responses (Figure 6B). Moreover, in case of sustained responses there is a biphasic distribution in the saturation concentrations with one peak around 0.1 pM and the other one around 10 pM (Figure 6C). However, in both transient and sustained cases, the transcription factor is able to reach similar maximal values (Additional file 3, Fig. S8). On the contrary, the maximal output value reached by oscillating responses is much lower than in the sustained and transient case. Our results are mostly in agreement with the conclusions drawn by Chung et al. (2009), who showed also that transient TGF-β responses saturate. However, deviating from our results, Chung and co-workers observed that also in transient responses the peak value is reached more rapidly as the stimulus concentration increases.
For parameter sets that give rise to oscillatory responses, changing the input strength and shape does not influence the period of oscillation but modulates the evolution of the oscillations amplitudes (data not shown). When exposed to sustained, high TGF-β concentrations the amplitude of oscillations starts to decay from the beginning. When the TGF-β concentration raises progressively, the amplitude of oscillation first raises and then decays, reflecting two competing phenomena: the amplitude of oscillations tends to be proportional to the input, but at the same time the sequestration of the receptor by the inhibitor leads to a dampening of the amplitude.
We next investigated in how far the kinetic parameters can influence the saturation concentration (Figure 6B, C) and the maximal output value at saturation (Additional file 3, Fig. S8 and Additional file 3, Fig. S9). For transient responses it is mainly the rate of ligand-receptor binding, k2, that determines the saturation concentration (Figure 6D and Additional file 3, Fig. S10). In case of slow binding higher concentrations of ligand are required to saturate the receptors. The saturation concentration for sustained responses are determined both by the receptor-ligand binding rate, k2, and by the cytoplasm-nucleus shuttling rate, k8 (Figure 6E and Additional file 3, Fig. S10). Fast shuttling enables more rapid deactivation of Smads as based on observations by Hill and coworkers  dephosphorylation is restricted to the nucleus in our model. As discussed above k2 and k8 have both been reported to be modulated by other processes. The saturation concentration can therefore also be adjusted by cross-talk.
The different saturation concentrations are likely important for the TGF-β response as different genes can be activated or repressed depending on the nuclear Smad complex concentration. While the mechanism by which different concentrations of the nuclear transcription factor complex translate into different transcriptional responses has not been resolved, likely mechanisms include promotor selection based on differences in the promoter binding-site affinities, cross-repression, and the establishment of a reciprocal of repressor gene expression [36, 37].
Proportional "faithful" responses
When ligands of the TGF-β family act as a morphogen, as it is for example the case for Dpp in Drosophila or Activin in Xenopus, cells must finely sense extracellular concentrations and transduce this signal inside the cell. We therefore looked for parameter sets leading to a response proportional to the input which we term "faithful". The parameter sets that gave rise to anything but sustained responses (i.e. transient, oscillatory, non-responsive, undefined responses) to sustained ligand exposure can already be discarded. Those parameter sets that gave rise to sustained responses to sustained ligand exposure we sought to analyse further with dynamic input signals. Here we used as input a function that first linearly increased from 0 to 720 pM for 5 hours and then linearly decreased to zero over the next 5 hours (Figure 7A). To screen our simulations for "faithful" parameter sets we normalized both the input and the output with respect to their respective highest values, and calculated the squared residuals R between input and output according to . The 10% sets with the lowest residual were classified as "faithful" and the 10% sets with the highest residual were classified as "unfaithful" for further analysis (Additional file 3, Fig. S11).
A response is faithful if the output is proportional to the input over time, i.e. y output (t) = α × y input (t), where α is the proportionality coefficient. This requires (i) that the output adapts rapidly to changes in the input, and (ii) that the response does not saturate, i.e. max(y output (t)) < max(O peak ), which is the case if the proportionality coefficient α is low and/or the maximal response value max(O peak ) is high. Those requirements are reflected in the constraints on the parameter values (Figure 7B-C) for faithful responses, i.e. a low binding rate of TGF-β to its receptor and a low phosphorylation rate prevent early saturation of the output, while a relative weak feedback and a low binding rate of the I-Smad to the receptor prevent a premature termination of the response. We have previously discussed the regulation of the binding rate of TGF-β to its receptor, k2 and thus now focus on the feedback. The different I-Smads have been shown to vary in their effects. Thus Dad, the Drosophila I-Smad, appears to interfere mainly with the BMP-like pathways (Tkv and Sax receptor dependent pathways) but not the Activin-like Babo-dependent pathway . Inhibition by vertebrate Smad6 and Smad7 can be achieved by sequestration, enhanced degradation, or an impact on phosphorylation. The different processes likely have different efficiencies and this will determine the efficiency of the negative feedback.
Our results indicate that under certain parameter restrictions the extracellular concentration is directly reflected in the output concentration. In that case, TGF-β can act as a morphogen, conveying positional information and determining cell-fate, subjected to the set of activated and repressed genes.
The duration of the signaling response is thought to be an important factor influencing the cell's phenotypic response to TGF-β. We have employed a very simple model of the TGF-β network to better understand the mechanistic basis of the observed signaling plasticity. We find that the qualitative response (transient, sustained, oscillations, proportional responses) to a constant ligand exposure can indeed be changed by altering the value of a single parameter value. Since we consider a simple model each parameter value represents a wider range of processes and our observation thus implies that both changes in protein concentration as well as cross-talk between signaling pathways can alter the qualitative response to a TGF-β stimulus. Many more complicated models for TGF-β signaling as well as for other signaling networks have been proposed already. To better understand the regulatory impact of cross-talk it will be important to connect experimentally validated models for the TGF-β network also to those for other pathway models. While many kinetic parameters have been measured an important parameter that remains often unmeasured is the protein concentrations. To better predict the responses in different cell types it will be important to obtain quantitative information on protein abundance in different cell types - and eventually in individual cells.
Blobe GC, Schiemann WP, Lodish HF: Role of transforming growth factor beta in human disease. N Engl J Med. 2000, 342 (18): 1350-8. 10.1056/NEJM200005043421807.
Shi Y, Massague J: Mechanisms of TGF-beta signaling from cell membrane to the nucleus. Cell. 2003, 113 (6): 685-700. 10.1016/S0092-8674(03)00432-X.
Schmierer B, Tournier AL, Bates PA, Hill CS: Mathematical modeling identifies Smad nucleocytoplasmic shuttling as a dynamic signal-interpreting system. Proc Natl Acad Sci USA. 2008, 105 (18): 6608-13. 10.1073/pnas.0710134105.
Pierreux CE, Nicolas FJ, Hill CS: Transforming growth factor beta-independent shuttling of Smad4 between the cytoplasm and nucleus. Mol Cell Biol. 2000, 20 (23): 9041-54. 10.1128/MCB.20.23.9041-9054.2000.
Kang Y, Chen CR, Massague J: A self-enabling TGFbeta response coupled to stress signaling: Smad engages stress response factor ATF3 for Id1 repression in epithelial cells. Mol Cell. 2003, 11 (4): 915-26. 10.1016/S1097-2765(03)00109-6.
Kavsak P, Rasmussen RK, Causing CG, Bonni S, Zhu H, Thomsen GH, Wrana JL: Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF beta receptor for degradation. Mol Cell. 2000, 6 (6): 1365-75. 10.1016/S1097-2765(00)00134-9.
Ebisawa T, Fukuchi M, Murakami G, Chiba T, Tanaka K, Imamura T, Miyazono K: Smurf1 interacts with transforming growth factor-beta type I receptor through Smad7 and induces receptor degradation. J Biol Chem. 2001, 276 (16): 12477-80. 10.1074/jbc.C100008200.
Randall RA, Germain S, Inman GJ, Bates PA, Hill CS: Different Smad2 partners bind a common hydrophobic pocket in Smad2 via a defined proline-rich motif. EMBO J. 2002, 21 (1-2): 145-56.
Hayashi H, Abdollah S, Qiu Y, Cai J, Xu YY, Grinnell BW, Richardson MA, Topper JN, Gimbrone JMA, Wrana JL, Falb D: The MAD-related protein Smad7 associates with the TGFbeta receptor and functions as an antagonist of TGFbeta signaling. Cell. 1997, 89 (7): 1165-73. 10.1016/S0092-8674(00)80303-7.
Massague J, Chen YG: Controlling TGF-beta signaling. Genes Dev. 2000, 14 (6): 627-44.
Esparza-Lopez J, Montiel JL, Vilchis-Landeros MM, Okadome T, Miyazono K, Lopez-Casillas F: Ligand binding and functional properties of betaglycan, a co-receptor of the transforming growth factor-beta superfamily. Specialized binding regions for transforming growth factor-beta and inhibin A. J Biol Chem. 2001, 276 (18): 14588-96. 10.1074/jbc.M008866200.
Di Guglielmo GM, Le Roy C, Goodfellow AF, Wrana JL: Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover. Nat Cell Biol. 2003, 5 (5): 410-21. 10.1038/ncb975.
Pyrowolakis G, Hartmann B, Muller B, Basler K, Affolter M: A simple molecular complex mediates widespread BMP-induced repression during Drosophila development. Dev Cell. 2004, 7 (2): 229-40. 10.1016/j.devcel.2004.07.008.
Moustakas A, Souchelnytskyi S, Heldin CH: Smad regulation in TGF-beta signal transduction. J Cell Sci. 2001, 114 (Pt 24): 4359-69.
Kretzschmar M, Doody J, Timokhina I, Massague J: A mechanism of repression of TGFbeta/Smad signaling by oncogenic Ras. Genes Dev. 1999, 13 (7): 804-16. 10.1101/gad.13.7.804.
Wicks SJ, Lui S, Abdel-Wahab N, Mason RM, Chantry A: Inactivation of smad-transforming growth factor beta signaling by Ca(2+)-calmodulin-dependent protein kinase II. Mol Cell Biol. 2000, 20 (21): 8103-11. 10.1128/MCB.20.21.8103-8111.2000.
Matsuura I, Denissova NG, Wang G, He D, Long J, Liu F: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature. 2004, 430 (6996): 226-31. 10.1038/nature02650.
Grimm OH, Gurdon JB: Nuclear exclusion of Smad2 is a mechanism leading to loss of competence. Nat Cell Biol. 2002, 4 (7): 519-22. 10.1038/ncb812.
Clarke DC, Liu X: Decoding the quantitative nature of TGF-beta/Smad signaling. Trends Cell Biol. 2008, 18 (9): 430-42. 10.1016/j.tcb.2008.06.006.
Clarke DC, Betterton MD, Liu X: Systems theory of Smad signalling. Syst Biol (Stevenage). 2006, 153 (6): 412-24. 10.1049/ip-syb:20050055.
Nicolas FJ, Hill CS: Attenuation of the TGF-beta-Smad signaling pathway in pancreatic tumor cells confers resistance to TGF-beta-induced growth arrest. Oncogene. 2003, 22 (24): 3698-711. 10.1038/sj.onc.1206420.
Melke P, Jonsson H, Pardali E, ten Dijke P, Peterson C: A rate equation approach to elucidate the kinetics and robustness of the TGF-beta pathway. Biophys J. 2006, 91 (12): 4368-80. 10.1529/biophysj.105.080408.
Zi Z, Klipp E: Constraint-based modeling and kinetic analysis of the Smad dependent TGF-beta signaling pathway. PLoS One. 2007, 2 (9): e936-10.1371/journal.pone.0000936.
Chung SW, Miles FL, Sikes RA, Cooper CR, Farach-Carson MC, Ogunnaike BA: Quantitative modeling and analysis of the transforming growth factor beta signaling pathway. Biophys J. 2009, 96 (5): 1733-50. 10.1016/j.bpj.2008.11.050.
Zi Z, Feng Z, Chapnick DA, Dahl M, Deng D, Klipp E, Moustakas A, Liu X: Quantitative analysis of transient and sustained transforming growth factor-beta signaling dynamics. Mol Syst Biol. 2011, 7: 492-
Novák B, Tyson JJ: Design principles of biochemical oscillators. Nat Rev Mol Cell Biol. 2008, 9 (12): 981-991. 10.1038/nrm2530.
Shankaran H, Ippolito DL, Chrisler WB, Resat H, Bollinger N, Opresko LK, Wiley HS: Rapid and sustained nuclear-cytoplasmic ERK oscillations induced by epidermal growth factor. Molecular systems biology. 2009, 5: 332-
Paulsen M, Legewie S, Eils R, Karaulanov E, Niehrs C: Negative feedback in the bone morphogenetic protein 4 (BMP4) synexpression group governs its dynamic signaling range and canalizes development. PNAS. 2011
Vilar JM, Jansen R, Sander C: Signal processing in the TGF-beta superfamily ligand-receptor network. PLoS Comput Biol. 2006, 2: e3-10.1371/journal.pcbi.0020003.
Geier F, Fengos G, Iber D: A computational analysis of the dynamic roles of talin, Dok1, and PIPKI for integrin activation. PloS One. 2011.
Ma W, Trusina A, El-Samad H, Lim Wa, Tang C: Defining network topologies that can achieve biochemical adaptation. Cell. 2009, 138 (4): 760-73. 10.1016/j.cell.2009.06.013.
Shankaran H, Ippolito DL, Chrisler WB, Resat H, Bollinger N, Opresko LK, Wiley HS: Rapid and sustained nuclear-cytoplasmic ERK oscillations induced by epidermal growth factor. Mol Syst Biol. 2009, 5: 332-
Hata A, Lagna G, Massagué J, Hemmati-Brivanlou A: Smad6 inhibits BMP/Smad1 signaling by specifically competing with the Smad4 tumor suppressor. Genes Dev. 1998, 12 (2): 186-197. 10.1101/gad.12.2.186.
Wu MY, Hill CS: Tgf-beta superfamily signaling in embryonic development and homeostasis. Dev Cell. 2009, 16 (3): 329-343. 10.1016/j.devcel.2009.02.012.
Clarke DC, Brown ML, Erickson Ra, Shi Y, Liu X: Transforming growth factor beta depletion is the primary determinant of Smad signaling kinetics. Molecular and cellular biology. 2009, 29 (9): 2443-55. 10.1128/MCB.01443-08.
Schmierer B, Hill CS: TGFbeta-SMAD signal transduction: molecular specificity and functional flexibility. Nat Rev Mol Cell Biol. 2007, 8 (12): 970-82. 10.1038/nrm2297.
Ashe HL, Briscoe J: The interpretation of morphogen gradients. Development. 2006, 133 (3): 385-94. 10.1242/dev.02238.
Kamiya Y, Miyazono K, Miyazawa K: Specificity of the inhibitory effects of Dad on TGF-beta family type I receptors, Thickveins, Saxophone, and Baboon in Drosophila. FEBS letters. 2008, 582 (17): 2496-2500. 10.1016/j.febslet.2008.05.052. [Kamiya, Yuto Miyazono, Kohei Miyazawa, Keiji Research Support, Non-U.S. Gov't Netherlands FEBS letters FEBS Lett. 2008 Jul 23;582(17):2496-500. Epub 2008 Jun 25.]
This research was supported by an iPhD SystemsX grant to Georgios Fengos.
DI and GF developed the model, GF and MH carried out an initial analysis of the model, GF developed the infrastructure for the parameter screens, GC carried out the analysis of the model, MH created Figure 1, GC and DI wrote the paper. All authors read and approved the final manuscript.
Geraldine Cellière, Georgios Fengos contributed equally to this work.
Electronic supplementary material
Additional file 2: Supplementary_Tables. Tables of the model parameters and the equations used in the model. (PDF 207 KB)
Additional file 3: Supplementary_Figures. Figures for the response classification of the parameter screen (Fig. S5), the dependance of the damping of oscillations on k2 (Fig. S6), boxplots for the parameter sets that can lead to both transient and sustained responses (Fig. S7), distribution of the maximal output value (Fig. S8), boxplots for parameters with high and low saturation value (Fig. S10), and with high and low maximal value (Fig. S9), boxplots for faithful and unfaithful parameters sets (Fig. S11), the evolution of the concentrations of all species in a representative cases (Fig. S1, S2, S3 and S4). (PDF 2 MB)
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Cellière, G., Fengos, G., Hervé, M. et al. The plasticity of TGF-βsignaling. BMC Syst Biol 5, 184 (2011). https://doi.org/10.1186/1752-0509-5-184
- Transient Response
- Response Type
- Ligand Concentration
- Sustained Response
- Sustained Oscillation